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首页> 外文期刊>Sensors and Actuators >Development of a high sensitive automatic setup for screening of microcystins in surface waters by employing a LED-based photometric detector
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Development of a high sensitive automatic setup for screening of microcystins in surface waters by employing a LED-based photometric detector

机译：通过使用基于LED的光度检测器，开发了一种用于筛选地表水中微囊藻毒素的高灵敏度自动装置

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摘要

In this manuscript, an automatic setup for screening of microcystins in surface waters by employing photometric detection is described. Microcystins are toxins delivered by cyanobacteria within an aquatic environment, which have been considered strongly poisonous for humans. For that reason, the World Health Organization (WHO) has proposed a provisional guideline value for drinking water of 1 μg L~(-1). In this work, we developed an automated equipment setup, which allows the screening of water for concentration of microcystins below 0.1 μg L~(-1). The photometric method was based on the enzyme-linked immunosorbent assay (ELISA) and the analytical signal was monitored at 458 nm using a homemade LED-based photometer. The proposed system was employed for the detection of microcystins in rivers and lakes waters. Accuracy was assessed by processing samples using a reference method and applying the paired t-test between results. No significant difference at the 95% confidence level was observed. Other useful features including a linear response ranging from 0.05 up to 2.00 μg L~(-1) (R~2 =0.999) and a detection limit of 0.03 μg L~(-1) microcystins were achieved.
机译：在此手稿中，描述了通过采用光度检测来筛选地表水中微囊藻毒素的自动装置。微囊藻毒素是蓝藻在水生环境中传递的毒素，被认为对人有剧毒。因此，世界卫生组织（WHO）提出了关于饮用水的暂行准则值1μgL〜（-1）。在这项工作中，我们开发了一种自动化的设备设置，可以筛查水中微囊藻毒素的浓度是否低于0.1μgL〜（-1）。光度法基于酶联免疫吸附测定（ELISA），并使用自制的基于LED的光度计在458 nm处监测分析信号。拟议的系统被用于检测河流和湖泊水域中的微囊藻毒素。通过使用参考方法处理样品并在结果之间应用配对t检验来评估准确性。在95％置信水平下未观察到显着差异。其他有用的功能包括线性响应范围从0.05到2.00μgL〜（-1）（R〜2 = 0.999）和检测限为0.03μgL〜（-1）微囊藻毒素。

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