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首页> 外文期刊>Sensors and Actuators >Simple disposable microfluidic device for Salmonella typhimurium detection by magneto-immunoassay
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Simple disposable microfluidic device for Salmonella typhimurium detection by magneto-immunoassay

机译：磁免疫法检测鼠伤寒沙门氏菌的简易一次性微流控装置

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摘要

Herein, we describe the construction of a simple, low-cost, and fully disposable microfluidic device (DµFD) for ultrasensitive Salmonella typhimurium (S. typhi) detection. The DµFD is based on unmodified carbon electrode array for rapid detection of S. typhi in milk samples by magneto-immunoassay using gold nanoparticles (AuNPs) as a label. In the method proposed, S. typhi was magnetically captured and separated from samples using magnetic beads modified with a monoclonal anti-Salmone/(a antibody followed by the addition of polyclonal anti-Salmonella antibody labeling with AuNPs. The magneto-immunoconjugate formed was injected into the DµFD and captured on the electrode surface by placing magnets behind the unmodified working electrodes. The S. typhi detection was carried out by the electrochemical response of the gold from AuNPs. A single DµFD was used for rapid and full S. typhi analysis by measuring standards and samples simultaneously. A low limit of detection (LOD) of 7.7 cells mL~(-1) for S. typhi in a linear range from 10.0 to 100.0 cells mL~(-1) was obtained. The DµFD proved to be fast, accurate, and inexpensive for S. typhi detection and has great potential to multiplex detection of different food contaminants.
机译：本文中，我们描述了一种用于超灵敏鼠伤寒沙门氏菌（S. typhi）检测的简单，低成本且完全可抛弃的微流控设备（DµFD）的构造。 DµFD基于未经修饰的碳电极阵列，可通过使用金纳米颗粒（AuNPs）作为标记的磁免疫测定法快速检测牛奶样品中的伤寒沙门氏菌。在提出的方法中，使用单克隆抗Salmone /（a抗体）修饰的磁珠磁捕获伤寒沙门氏菌并从样品中分离，然后添加用AuNPs标记的多克隆抗Salmonella抗体。注入形成的磁免疫缀合物进入DµFD中，并通过在未修饰的工作电极后面放置磁铁将其捕获在电极表面上，通过AuNPs中金的电化学反应进行了伤寒沙门氏菌的检测，单个DµFD用于快速，完整的伤寒沙门氏菌分析。同时测定标准品和样品，得到的伤寒沙门氏菌的检出限（LOD）在10.0至100.0个细胞mL〜（-1）的线性范围内，为7.7细胞mL〜（-1）。快速，准确，廉价用于伤寒沙门氏菌检测，并具有多重检测不同食品污染物的巨大潜力。

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