...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>IEEE sensors journal >A Rapid and Sensitive Impedance-Based Immunosensor Utilizing Dielectrophoretic Manipulations of Polyaniline Modified Nanoprobes
【24h】

A Rapid and Sensitive Impedance-Based Immunosensor Utilizing Dielectrophoretic Manipulations of Polyaniline Modified Nanoprobes

机译：一种快速灵敏的基于阻抗的免疫传感器，利用聚苯胺修饰的纳米探针的介电泳操作。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this paper, we have fabricated a simple impedimetric bioanalytical device for the sensitive label free detection of specific immunoreactions. The device consists of a gold interdigitated microelectrode array that utilizes a positive dielectrophoretic (DEP) force to focus and concentrate the nanoprobes (conductive PANI-coated AlO nanoparticles conjugated to antibody). A novel surface modification technique has been used to obtain a strongly adhered coating while antibody immobilization occurs via covalent bonding between the amino groups on the PANI surface and the aldehyde groups of the binder, glutaraldehyde. As proof of concept, we have used this immunosensor for detecting the immunoreaction of bovine serum albumin (BSA) with antibovine serum albumin. We have compared the sensitivity of the device at different concentrations of BSA ranging from 1 nM to , with and without using DEP. Results show that the impedance change is directly proportional to the concentration of BSA used and can be magnified to about 2–3 times using DEP, thus providing an effective means for immobilization of the nanoprobes and improving the overall sensor sensitivity. Experimental impedance results match well with simulated results obtained using an equivalent circuit model. Thus, this disposable lab-on-a-chip device can be used in clinical settings for the quantitative detection of immunoassays with results obtainable within an hour.
机译：在本文中，我们制造了一种简单的阻抗分析生物分析设备，用于无特异性检测免疫反应的敏感标签。该设备由一个金指状微电极阵列组成，该阵列利用正介电（DEP）力聚焦和浓缩纳米探针（与抗体结合的导电PANI涂层AlO纳米粒子）。一种新颖的表面改性技术已用于获得牢固粘附的涂层，而抗体固定化是通过PANI表面上的氨基与粘合剂戊二醛的醛基之间的共价键发生的。作为概念验证，我们已使用该免疫传感器检测牛血清白蛋白（BSA）与抗牛血清白蛋白的免疫反应。我们比较了在使用和不使用DEP的情况下，在1nM至1nM的不同BSA浓度下设备的灵敏度。结果表明，阻抗变化与所用BSA的浓度成正比，并且使用DEP可以放大到2-3倍，从而为固定纳米探针和提高整体传感器灵敏度提供了一种有效的方法。实验阻抗结果与使用等效电路模型获得的模拟结果非常吻合。因此，这种一次性的单芯片实验室设备可用于临床环境中以定量检测免疫测定，并在一小时内可获得结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号