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首页> 外文期刊>IEEE sensors journal >Plasmonic Sensing of Biological Analytes Through Nanoholes
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Plasmonic Sensing of Biological Analytes Through Nanoholes

机译:通过纳米孔的生物分析物的等离子传感。

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摘要

A transmission-based surface plasmon resonance (SPR) sensor for label-free detection of protein–carbohydrate and protein–protein binding proximate to a perforated gold surface is demonstrated. An SPR instrument makes real-time measurements of the resonant wavelength and/or the resonant angle of incidence of transmitted light; both are influenced by the presence of proteins at the gold surface-liquid interface. Ethylene glycol solutions with known refractive indexes were used to calibrate the instrument. A paired polarization-sensitive detector achieved an overall detection resolution of $sim {hbox{6.6}}times {hbox{10}}^{-5}$ refractive index units (RIU). Proof of principle experiments was performed with concanavalin A (Con A) binding to gold-adsorbed ovomucoid and anti-bovine serum albumin (BSA) binding to gold-adsorbed BSA.
机译:展示了一种基于透射的表面等离振子共振(SPR)传感器,该传感器可用于无标记地检测蛋白质–碳水化合物和蛋白质–蛋白质与穿孔金表面的结合。 SPR仪器可实时测量透射光的共振波长和/或共振入射角;两者都受金表面-液体界面处蛋白质的存在的影响。使用已知折射率的乙二醇溶液校准仪器。一对偏振敏感检测器的整体检测分辨率达到了$ sim {hbox {6.6}}乘以{hbox {10}} ^ {-5} $折射率单位(RIU)的水平。使用伴刀豆球蛋白A(Con A)结合到金吸附的卵类粘液和抗牛血清白蛋白(BSA)结合到金吸附的BSA进行原理实验的证明。

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