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首页> 外文期刊>IEEE sensors journal >Total Internal Reflection Fluorescence Foldscope Adapter: Design, Fabrication and Applications
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Total Internal Reflection Fluorescence Foldscope Adapter: Design, Fabrication and Applications

机译：全内部反射荧光折叠型转换适配器：设计，制造和应用

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Fluorescence microscopy is one of the important instruments used in research and clinical laboratories on an everyday basis due to its wide range of applications ranging from a diagnostic tool to a molecular imaging device. However, the high cost, complex instrumentation, and requirement of sophisticated facilities limit its availability to school and college students, especially in developing and underdeveloped countries. In this work, we report the design and development of a slide-like portable total internal reflection fluorescence (TIRF) adapter, which can be integrated easily with Foldscope (a paper microscope) to observe and capture the fluorescence images using any smartphone. The adapter consists of a battery-poweredLED(light source), asmall piece of multimode optical fiber, a slide-like 3-D printed waveguide platform to hold the optical fiber and light emitting diode (LED) at an appropriate position. The potential of the adaptor is validated by capturing the fluorescence images of two most commonly used dyes i.e. fluorescein isothiocyanate (FITC) and rhodamine-B, after being immobilized on aminefunctionalized optical fiber probe. Further, this adaptor is successfully used to demonstrate (i) Forster resonance energy transfer phenomenon by employing FITC dye as donor and gold nanoparticles as quencher moiety and (ii) fluorescence bioassay by observing antigen-antibody interactions using human immunoglobulin G as the receptor and FITC-tagged goat anti-human immunoglobulin G as analyte. The inexpensive, portable and user-friendly TIRF adapter is a potential alternative to conventionally used fluorescence microscopes, especially for the school and college students and in resource-constrained clinical laboratories.
机译：荧光显微镜是由于其广泛的应用范围从诊断工具到分子成像装置的应用而在日常基础上用于研究和临床实验室中的重要仪器之一。然而，高成本，复杂的仪器和精致设施的要求限制了学校和大学生的可用性，特别是在开发和欠发达国国家。在这项工作中，我们报告了类似智能手机的幻灯片（纸张显微镜）可以轻松地集成的平面便携式全内部反射荧光（TIRF）适配器的设计和开发，以便使用任何智能手机观察和捕获荧光图像。适配器由电池供电（光源），ASMALL块多模光纤，滑动三维印刷波导平台，以将光纤和发光二极管（LED）保持在适当位置。通过在固定在氨官能化光纤探针上的荧光素异硫氰酸酯（FITC）和罗丹明-B之后，通过捕获两个最常用的染料的荧光图像来验证适配器的电位。此外，通过使用人免疫球蛋白G作为受体和FITC的抗原 - 抗体相互作用，通过使用人免疫球蛋白G作为受体和FITC，通过观察抗原 - 抗体相互作用来证明（i）福尔斯特共振能量转移现象和荧光生物测定，通过观察使用人免疫球蛋白G作为受体和FITC，通过观察抗原 - 抗体相互作用来证明（i）福尔斯特共振能量转移现象作为猝灭剂部分和（ii）荧光生物测定 - 作为分析物 - 山羊抗人免疫球蛋白G.廉价，便携和用户友好的TIRF适配器是常规使用荧光显微镜的潜在替代品，特别是对于学校和大学生以及资源受限的临床实验室。

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