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首页> 外文期刊>Soil Science and Plant Nutrition >Differences in root-to-shoot Cd and Zn translocation and by HMA3 and 4 could influence chlorophyll and anthocyanin content in Arabidopsis Ws and Col-0 ecotypes under excess metals
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Differences in root-to-shoot Cd and Zn translocation and by HMA3 and 4 could influence chlorophyll and anthocyanin content in Arabidopsis Ws and Col-0 ecotypes under excess metals

机译：在过量金属下，从根到茎的镉和锌易位以及HMA3和4的差异可能影响拟南芥Ws和Col-0生态型中叶绿素和花色苷的含量

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Judging from the ecotypic variability in Arabidopsis thaliana L., Columbia-0 (Col-0) appears to be less tolerant to cadmium (Cd) than the Wassilewskija (Ws) ecotype that possesses the full-length Heavy Metal ATPase3 (HMA3) cDNA. In this study, the Ws and Col-0 were tested to determine toxic metal response between Ws and Col-0 due to AtHMA3 point mutation and/or other factors. The growth inhibition of Col-0 mediated by Cd and zinc (Zn) was more serious than the inhibition of Ws, while no significant difference was evident by lead (Pb) and cobalt (Co). In the presence of Cd stress, chlorosis in leaves of Col-0 was more serious than the Ws ecotype. When grown under hydroponic culture containing 500a???μM Zn, leaves of Col-0 showed a remarkable increase in the anthocyanin content in a dose-dependent manner and the expression of genes encoding enzymes involved in anthocyanin synthesis in the leaves. The rate of root-to-shoot translocation of Cd and Zn in the Col-0 was 2 times higher when compared with the Ws, whereas roots of the Col-0 accumulated 2 times lower Cd and Zn concentrations than those of the Ws. Real-time polymerase chain reaction (PCR) analyses indicated that not only the alteration of the expression of HMA3 but also of the HMA4 was responsible for the root-to-shoot translocation of toxic metals. The results demonstrate that the Col-0 is readily translocating Cd and Zn to the aerial parts but not the Ws, thereby induce the alteration of phenotype in leaf color.View full textDownload full textKeywordsanthocyanin, cadmium, heavy metal ATPase, point mutation, translocation rate, zinc.Related var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/00380768.2012.684643
机译：从拟南芥的生态型变异性来看，与拥有全长重金属ATPase3（HMA3）cDNA的Wassilewskija（Ws）生态型相比，Columbia-0（Col-0）对镉（Cd）的耐受性差。在这项研究中，测试了Ws和Col-0，以确定由于AtHMA3点突变和/或其他因素引起的Ws和Col-0之间的有毒金属反应。 Cd和锌（Zn）对Col-0的生长抑制作用比Ws的抑制作用更为严重，而铅（Pb）和钴（Co）的变化无明显差异。在Cd胁迫下，Col-0叶片的萎黄病比Ws生态型严重。当在含有500a？μMZn的水培培养物中生长时，Col-0的叶子显示出花青素含量以剂量依赖的方式显着增加，并且在叶子中编码参与花青素合成的酶的基因表达。与Ws相比，Col-0中Cd和Zn的根到茎易位率高2倍，而Col-0的根中Cd和Zn浓度比Ws低2倍。实时聚合酶链反应（PCR）分析表明，不仅HMA3表达的改变，而且HMA4的发生都导致有毒金属从根到茎的易位。结果表明，Col-0易于将Cd和Zn转移到地上部分而不是Ws，从而诱导叶片颜色表型的改变。查看全文下载全文关键词花色苷，镉，重金属ATPase，点突变，易位率，锌。相关的var addthis_config = {ui_cobrand：“泰勒和弗朗西斯在线”，servicescompact：“ citeulike，netvibes，twitter，technorati，delicious，linkedin，facebook，stumbleupon，digg，google，更多”，发布编号：“ ra-4dff56cd6bb1830b” };添加到候选列表链接永久链接http://dx.doi.org/10.1080/00380768.2012.684643

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