Identification and Phylogenetic Analysis of Xanthomonas albilineans (Ashby) Dowson Based on Multiple Gene Sequences in Yunnan Province, China

Zhang Rong-Yue; Wang Xiao-Yan; Shan Hong-Li; Li Jie; Li Wen-Feng; Cang Xiao-Yan; Luo Zhi-Ming; Yin Jiong; Huang Ying-Kun

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Identification and Phylogenetic Analysis of Xanthomonas albilineans (Ashby) Dowson Based on Multiple Gene Sequences in Yunnan Province, China

机译：基于云南省云南省多基因序列的Xanthomonas Albilina（Ashby）Dowson的鉴定和系统发育分析

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Sugarcane leaf scald caused by Xanthomonas albilineans (Ashby) Dowson is an extremely destructive disease. In 2017, suspected symptoms of sugarcane leaf scald was observed, in Baoshan, Mengding and Jinping, Yunnan Province, China. The bacteria was isolated from the symptomatic stalks and was subsequently identified as the causal agent using Koch's postulates. BLASTN analysis showed that the amplified nucleotide sequences of 16S rRNA, gyrB and rpoD genes from all isolates shared 100% sequence identity with the nucleotide sequences of corresponding genes of X. albilineans strain GPE PC73 (Acc no. FP565176). Based on symptom diagnosis in the field, colony morphology, validation of Koch's postulates and molecular identification of 16S rRNA, gyrB and rpoD genes, the disease was confirmed as sugarcane leaf scald caused by X. albilineans. Partial 16S rRNA, gyrB and rpoD gene sequence analysis of 36 X. albilineans strains from Yunnan and Guangxi provinces, China, was determined. The results revealed that the gyrB gene was the most sensitive in differentiating X. albilineans strains from Yunnan and Guangxi provinces.

机译：由Xanthomonas albilineans（Ashby）Dowson引起的甘蔗叶烫伤是一种极其破坏性的疾病。 2017年，观察到甘蔗叶烫伤的疑似症状，在中国宝山，蒙山，云南省云南省。细菌与症状茎分离，随后被鉴定为使用Koch假设的因果剂。 BLASTN分析表明，来自所有分离株的16S rRNA，陀螺和RPOD基因的扩增核苷酸序列与X.bilineans菌株GPE PC73（ACC NO.FP565176）的相应基因的核苷酸序列共享100％序列同一性。基于现场的症状诊断，菌落形态，KOCH的假设和分子鉴定16S rRNA，GYRB和RPOD基因的验证，该疾病被证实为X. Albilineans引起的甘蔗叶烫伤。确定了云南和广西省份36 X.BaliNeans菌株的部分16S rRNA，GyrB和RPOD基因序列分析。结果表明，GyrB基因对云南和广西省份的X.Balileins菌株分化最敏感。

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《Sugar Tech》 |2019年第5期|794-801|共8页
作者
Zhang Rong-Yue; Wang Xiao-Yan; Shan Hong-Li; Li Jie; Li Wen-Feng; Cang Xiao-Yan; Luo Zhi-Ming; Yin Jiong; Huang Ying-Kun;
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Yunnan Acad Agr Sci Sugarcane Res Inst Yunnan Key Lab Sugarcane Genet Improvement Kaiyuan 661699 Peoples R China;

Yunnan Acad Agr Sci Sugarcane Res Inst Yunnan Key Lab Sugarcane Genet Improvement Kaiyuan 661699 Peoples R China;

Yunnan Acad Agr Sci Sugarcane Res Inst Yunnan Key Lab Sugarcane Genet Improvement Kaiyuan 661699 Peoples R China;

Yunnan Acad Agr Sci Sugarcane Res Inst Yunnan Key Lab Sugarcane Genet Improvement Kaiyuan 661699 Peoples R China;

Yunnan Acad Agr Sci Sugarcane Res Inst Yunnan Key Lab Sugarcane Genet Improvement Kaiyuan 661699 Peoples R China;

Yunnan Acad Agr Sci Sugarcane Res Inst Yunnan Key Lab Sugarcane Genet Improvement Kaiyuan 661699 Peoples R China;

Yunnan Acad Agr Sci Sugarcane Res Inst Yunnan Key Lab Sugarcane Genet Improvement Kaiyuan 661699 Peoples R China;

Yunnan Acad Agr Sci Sugarcane Res Inst Yunnan Key Lab Sugarcane Genet Improvement Kaiyuan 661699 Peoples R China;

Yunnan Acad Agr Sci Sugarcane Res Inst Yunnan Key Lab Sugarcane Genet Improvement Kaiyuan 661699 Peoples R China;

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Sugarcane leaf scald; Xanthomonas albilineans (Ashby) Dowson; Phylogenetic analysis; Yunnan Province;

机译：甘蔗叶烫伤;Xanthomonas albilineans（ashby）dowson;系统发育分析;云南省;

Identification and Phylogenetic Analysis of Xanthomonas albilineans (Ashby) Dowson Based on Multiple Gene Sequences in Yunnan Province, China

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