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首页> 外文期刊>Sugar Tech >Isolation, Characterization and Promoter Analysis of Cell Wall Invertase Gene SoCIN1 from Sugarcane (Saccharum spp.)
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Isolation, Characterization and Promoter Analysis of Cell Wall Invertase Gene SoCIN1 from Sugarcane (Saccharum spp.)

机译：甘蔗（Saccharum spp。）细胞壁转化酶基因SoCIN1的分离，鉴定和启动子分析

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摘要

Cell wall invertase (CIN) plays an important role in carbohydrate partitioning and regulation of sink-source interaction. In this study, a full length cDNA encoding CIN gene was cloned by RT-PCR and RACE-PCR from sugarcane. The open reading frame of 1731 bp encodes a protein of 577 amino acids with a predicted molecular mass of 141.04 kDa and theoretical PI of 4.67. A size of 4,537 bp DNA encoding SoCIN1 was isolated, which contains seven exons and six introns. Using genome walking approach, a promoter sequence of 974 bp of SoCIN1 was isolated, and prediction with PlantCARE and PLACE software revealed that it has several key cis-reg-ulatory elements known to be involved in various biotic and abiotic plant stresses. Relative expression in specific tissues was done using qRT-PCR, and the results demonstrated that SoCIN1 expression level was higher in the immature leaves and maturing leaves than the matured leaves and internodes at elongation stage and processing maturing stage. The conditions of 15 % PEG and 6 ℃ could have a similar function for inducing the expression of SoCIN gene in leaves, while 15 % PEG, 100 mM NaCl and 6℃ could induce the expression of SoCIN gene in roots.
机译：细胞壁转化酶（CIN）在碳水化合物分配和调节水槽-源相互作用中起重要作用。在这项研究中，通过RT-PCR和RACE-PCR从甘蔗中克隆了编码CIN基因的全长cDNA。 1731 bp的开放阅读框编码577个氨基酸的蛋白质，预测分子量为141.04 kDa，理论PI为4.67。分离出大小为4,537 bp的编码SoCIN1的DNA，其中包含七个外显子和六个内含子。使用基因组步移方法，分离了974 bp的SoCIN1启动子序列，并通过PlantCARE和PLACE软件进行的预测表明，它具有几个关键的顺式调控元件，已知与多种生物和非生物植物胁迫有关。使用qRT-PCR在特定组织中进行相对表达，结果表明，在延伸阶段和加工成熟阶段，未成熟叶片和成熟叶片中SoCIN1的表达水平高于成熟叶片和节间。 15％PEG和6℃条件可能具有相似的诱导SoCIN基因在叶片中表达的功能，而15％PEG，100 mM NaCl和6℃可以诱导根中SoCIN基因表达。

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  • 来源
    《Sugar Tech》 |2015年第1期|65-76|共12页
  • 作者

    Jun-Qi Niu; Ai-Qin Wang; Jing-Li Huang; Li-Tao Yang; Yang-Rui Li;

  • 作者单位

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresource, College of Agriculture, Guangxi University, 100 East Daxue Road, Nanning 530005, China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresource, College of Agriculture, Guangxi University, 100 East Daxue Road, Nanning 530005, China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresource, College of Agriculture, Guangxi University, 100 East Daxue Road, Nanning 530005, China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresource, College of Agriculture, Guangxi University, 100 East Daxue Road, Nanning 530005, China,Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Nanning, People's Republic of China Sugarcane Research Center, Chinese Academy of Agricultural Sciences-Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, China;

    Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Nanning, People's Republic of China Sugarcane Research Center, Chinese Academy of Agricultural Sciences-Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, China;

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  • 正文语种 eng
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  • 关键词

    Sugarcane; Cell wall invertase; Gene expression; Promoter;

    机译：甘蔗;细胞壁转化酶;基因表达;发起人;

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