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首页> 外文期刊>TAG Theoretical and Applied Genetics >Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred
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Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred

机译：K0326Y优质蛋白玉米自交系产生的重组自交系opaque2修饰子QTL和候选基因的鉴定。

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摘要

Quality protein maize (QPM) is a high lysine-containing corn that is based on genetic modification of the opaque2 (o2) mutant. In QPM, modifier genes convert the starchy endosperm of o2 to the vitreous phenotype of wild type maize. There are multiple, unlinked o2 modifier loci (Opm) in QPM and their nature and mode of action are unknown. We previously identified seven Opm QTLs and characterized 16 genes that are differentially up-regulated at a significant level in K0326Y QPM, compared to the starchy endosperm mutant W64Ao2. In order to further characterize these Opm QTLs and the genes up-regulated in K0326Y QPM, we created a population of 314 recombinant inbred lines (RILs) from a cross between K0326Y QPM and W64Ao2. The RILs were characterized for three traits associated with endosperm texture: vitreousness, density and hardness. Genetic linkage analysis of the RIL population confirmed three of the previously identified QTLs associated with o2 endosperm modification in K0326Y QPM. Many of the genes up-regulated in K0326Y QPM showed substantially higher levels of expression in vitreous compared with opaque RILs. These included genes associated with the upstream regulation of the ethylene response pathway, and a gene encoding a regulatory subunit of pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase, an adaptive enzyme of the glycolytic pathway.
机译：优质蛋白玉米（QPM）是基于opaque2（o2）突变体的遗传修饰的高赖氨酸玉米。在QPM中，修饰基因将o2的淀粉胚乳转化为野生型玉米的玻璃体表型。 QPM中有多个未链接的o2修饰基因座（Opm），其作用和作用方式尚不清楚。我们先前鉴定了七个Opm QTL，并鉴定了与淀粉质胚乳突变体W64Ao2相比在K0326Y QPM中显着水平差异上调的16个基因。为了进一步表征这些Opm QTL和在K0326Y QPM中上调的基因，我们从K0326Y QPM和W64Ao2之间的杂交中创建了314个重组自交系（RIL）。 RIL具有与胚乳质地相关的三个特征：玻璃质，密度和硬度。 RIL群体的遗传连锁分析确认了K0326Y QPM中三个先前鉴定出的与o2胚乳修饰相关的QTL。与不透明的RIL相比，在K0326Y QPM中上调的许多基因在玻璃体中的表达水平明显更高。这些包括与乙烯应答途径的上游调节有关的基因，以及编码焦磷酸依赖性果糖-6-磷酸1-磷酸转移酶（一种糖酵解途径的适应性酶）的调节亚基的基因。

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