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首页> 外文期刊>Theoretical and Applied Genetics >Mapping QTLs influencing rice floral morphology using recombinant inbred lines derived from a cross between Oryza sativa L. and Oryza rufipogon Griff
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Mapping QTLs influencing rice floral morphology using recombinant inbred lines derived from a cross between Oryza sativa L. and Oryza rufipogon Griff

机译:利用重组水稻自交系杂交获得的水稻水稻形态学QTL图谱

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摘要

To understand the genetic basis of floral traits associated with the mating system in rice, we analyzed pistil, stamen and glume traits using a recombinant inbred line population, derived from a cross between an Asian cultivated rice (Oryza sativa L.), Pei-kuh, and a wild rice (Oryza rufipogon Griff.), W1944. Quantitative trait loci (QTLs) affecting floral morphology were detected by composite interval mapping using a linkage map constructed using 147 markers, mostly RFLPs. A total of 7, 4, 14 and 6 QTLs were detected for traits related to pistil, stamen, and size and shape of the glume, respectively. Comparison of 31 QTLs affecting these organs revealed ten QTLs affecting the different organs in four adjacent regions on chromosomes 2, 4, 5 and 10, but most QTLs (68%) were located separately on the whole chromosomes. Although four QTLs for stigma breadth, anther length and thickness of lemma and palea explained more than 25% of the total phenotypic variance, most QTLs (87%) had smaller effects. These results suggest that quantitative variation observed for pistil, stamen and glume traits is controlled by several distinct genes with small effects.
机译:为了了解与水稻交配系统相关的花卉性状的遗传基础,我们使用重组自交系群体分析了雌蕊,雄蕊和颖颖性状,该群体来自亚洲栽培稻(Oryza sativa L.)与Pei-kuh之间的杂交和野生稻(Oryza rufipogon Griff。),W1944。通过使用147个标记(主要是RFLP)构建的连锁图,通过复合区间作图法检测影响花卉形态的数量性状基因座(QTL)。共检出7、4、14和6个QTL,分别与雌蕊,雄蕊以及颖片的大小和形状有关。对影响这些器官的31个QTL的比较显示,有10个影响2、4、5和10号染色体上四个相邻区域中不同器官的QTL,但是大多数QTL(68%)分别位于整个染色体上。尽管有四个QTL可以用于柱头宽度,花药长度,内ma和内果的厚度解释超过总表型变异的25%,但大多数QTL(87%)的影响较小。这些结果表明,观察到的雌蕊,雄蕊和颖片性状的定量变化受几个不同基因的影响很小。

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