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首页> 外文期刊>Theoretical and Applied Genetics >A bentazon and sulfonylurea sensitive mutant: breeding, genetics and potential application in seed production of hybrid rice
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A bentazon and sulfonylurea sensitive mutant: breeding, genetics and potential application in seed production of hybrid rice

机译：苯达松和磺酰脲敏感性突变体：杂交水稻的育种，遗传及在种子生产中的潜在应用

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The use of a thermosensitive genic male sterility (TGMS) system in two-line hybrid rice breeding is affected greatly by the sterility instability of TGMS lines caused by temperature fluctuation beyond their critical temperatures for fertility reversion. To prevent seed production from self contamination, we have developed a system to secure seed purity using a herbicide-sensitive TGMS mutant, M8077S, obtained by radiation. Genetic analysis, using the F1, F2 and F3 populations derived from this mutant and other normal varieties, revealed that bentazon lethality/sensitivity was controlled by a single recessive gene, which was named bel. The mutant can be killed at the seedling stage by bentazon at 300 mg/l or higher, a dosage that is safe for its F1 hybrids and all other normal varieties. This mutant is also sensitive to all the tested sulfonylurea herbicides. Response of segregating plants to these two types of herbicide indicated that sulfonylurea sensitivity was also controlled by bel. By crossing this mutant with Pei-Ai 64S, an F2 population was developed for genetic mapping. Surveying the two DNA pools from sensitive and non-sensitive F2 plants identified four markers that were polymorphic between the pools. The putative linked markers were then confirmed with the F2 population. The bel locus was located on chromosome 3, 7.1 cM from the closest microsatellite marker RM168. Phenotypic analysis indicated that the bel gene had no negative effect on agronomic traits in either a homozygous or heterozygous status. The mutant M8077S is valuable in the development of a TGMS breeding system for preventing impurity resulting from temperature fluctuation of the TGMS. Several two-line hybrid rice crosses using this system are under development.
机译：在两系杂交水稻育种中使用热敏基因雄性不育（TGMS）系统会受到温度超出其繁殖力临界温度的温度波动引起的TGMS系不育不稳定性的极大影响。为了防止种子生产受到自我污染，我们开发了一种系统，该系统使用通过辐射获得的对除草剂敏感的TGMS突变体M8077S来确保种子纯度。遗传分析，使用该突变体和其他正常品种的F1 ，F2 和F3 种群，发现苯达松的致死性/敏感性由单个隐性基因控制，即bel 。该突变体在苗期可以通过苯达松以300 mg / l或更高的剂量杀死，该剂量对其F1杂种和所有其他正常品种均安全。该突变体还对所有测试的磺酰脲类除草剂敏感。隔离植物对这两种除草剂的反应表明，磺酰脲敏感性也受bel控制。通过将该突变体与Pei-Ai 64S杂交，开发了一个F2 种群进行遗传定位。对敏感和不敏感的F2 植物的两个DNA库进行调查，发现了四个标记在两个库之间是多态的。然后用F2 群体确认推定的连锁标记。 bel位点位于3号染色体，距最近的微卫星标记RM168 7.1 cM。表型分析表明，bel基因对纯合或杂合状态的农艺性状均无负面影响。突变体M8077S在开发TGMS育种系统方面很有价值，该系统可防止TGMS的温度波动而产生杂质。使用该系统的几个两系杂交水稻杂交正在开发中。

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