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首页> 外文期刊>Theoretical and Applied Genetics >Differentiation and inter-genomic relationships among C, E and D genomes in the Oryzaofficinalis complex (Poaceae) as revealed by multicolor genomic in situ hybridization
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Differentiation and inter-genomic relationships among C, E and D genomes in the Oryzaofficinalis complex (Poaceae) as revealed by multicolor genomic in situ hybridization

机译：多色基因组原位杂交揭示了稻草复合体（禾本科）中C，E和D基因组的分化和基因组间关系

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摘要

The multicolor genomic in situ hybridization (McGISH) method was used to study differentiation and relationships among the C, D and E genomes in the officinalis complex of the genus Oryza. The chromosomes of Oryza alta (CCDD genomes) were hybridized with labelled probes of the C genome (from diploid Oryza eichingeri and Oryza officinalis) and the E genome (from Oryza australiensis) simultaneously. By adjusting the post-hybri- dization washing stringency in a gradual series, differentiation between the genomes was detected according to the homology between the target genomes and the probes. The McGISH results indicate that the C, D and E genomes share a substantial amount of similar sequences, and differentiation between the D and C genomes of O. alta is less than that between the E genome and each of the C and D genomes. The differentiation within the C genomes of the diploid species (O. officinalis and O. eichingeri) and the C genome of O. alta was clearly discerned by McGISH, suggesting strongly that neither O. officinalis nor O. eichingeri was the direct C-genome donor of O. alta. The evidence of the GISH results also indicates that the E genome was considerably differentiated from the C and D genomes. Therefore, the E genome should not be the direct donor of O. alta; on the contrary, the E genome is closer to the C than to the D genome. McGISH is an efficient method in revealing the relationships among the genomes in question, particularly under the gradual stringent-washing condition.
机译：多色基因组原位杂交（McGISH）方法用于研究稻属药用复合物中C，D和E基因组的分化和相互关系。将Oryza alta（CCDD基因组）的染色体与C基因组（来自二倍体Oryza eichingeri和Oryza officinalis）和E基因组（来自Oryza australiensis）的标记探针同时杂交。通过逐步调整杂交后的洗涤严格性，可根据目标基因组与探针之间的同源性检测基因组之间的差异。 McGISH结果表明，C，D和E基因组共享大量相似序列，O。alta的D和C基因组之间的差异小于E基因组与C和D基因组之间的差异。 McGISH清楚地识别了二倍体物种（O. officinalis和O. eichingeri）的C基因组内的分化以及O. alta的C基因组，这强烈表明，O。officinalis和O. eichingeri都不是直接的C基因组O. alta的捐助者。 GISH结果的证据还表明，E基因组与C和D基因组有很大区别。因此，E基因组不应该是O. alta的直接捐助者。相反，E基因组比D基因组更接近C。 McGISH是揭示相关基因组之间关系的一种有效方法，尤其是在逐步严格洗涤条件下。

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  • 来源
    《Theoretical and Applied Genetics》 |2001年第3期|197-203|共7页
  • 作者

    C.-B. Li; D.-M. Zhang; S. Ge; B.-R. Lu; D.-Y. Hong;

  • 作者单位

    Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China e-mail: zhangdm@ns.ibcas.ac.cn;

    Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China e-mail: zhangdm@ns.ibcas.ac.cn;

    Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China e-mail: zhangdm@ns.ibcas.ac.cn;

    Genetic Resources Center International Rice Research Institute (IRRI) MCPO Box 3127 1271 Makati City The Philippines Current address: Institute of Biodiversity Science Fudan University Shanghai 200433 China;

    Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China e-mail: zhangdm@ns.ibcas.ac.cn;

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    Keywords Genomic in situhybridization (GISH); Oryza officinalis complex; C genome; E genome; CD genome;

    机译：基因组原位杂交;Oryza officinalis复合体;C基因组;E基因组;CD基因组;

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