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Effect of Pre-existing Dyes and Fabric Type on Cellulase Treatment of Cotton Fabrics

机译：既有染料和织物类型对棉织物纤维素酶处理的影响

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Fabric weight loss through cellulase treatment of six different cottons depends on mercerization, yarn size, and fabric structure. Weight loss decreases in the order of Ne = 60 mercerized > Ne = 60 nonmercerized ≈ Ne = 40 mercerized > Ne = 40 nonmercerized knit fabrics > Ne = 20 ≈ Ne = 10 nonmercerized woven fabrics, indicating increased enzymatic hydrolysis on mercerized cotton knits with smaller yarn size. The effect of pre-existing dyes on cellulase activity is examined by measuring the difference in weight reduction for mercerized and nonmercerized cotton knits previously dyed with direct, reactive, and vat dyes. All six dyes in this study inhibit the cellulase reaction, and the degree of inhibition depends on the dye class. The concentration of dyes on fabric as estimated by K/S proves to be the most critical factor governing resulting weight losses. The planar structure of vat dyes with larger molecular sizes than indigo dye seems to play an important role in their inhibitory effect. Compared with monoreactive, direct, and vat dyes, the largest inhibitory effect is with bifunctional dyes, implying that the crosslinking property of bifunctional dyes is related to cellulase hydrolysis. This suggestion is further supported by the small reduction in weight after cellulase treatment of DMDHEU-treated cotton fabric. A model is proposed to explain the slower progress of enzymatic cleavage on crosslinked cellulose fibers.
机译：通过纤维素酶处理六种不同的棉花可以减轻织物的重量，具体取决于丝光处理，纱线尺寸和织物结构。重量减轻的顺序为：Ne = 60丝光> Ne = 60丝光≈≈Ne = 40丝光> Ne = 40丝光的针织布> Ne = 20≈Ne = 10丝光的机织织物，表明较小丝光棉针织物的酶水解增加纱线尺寸。通过测量预先用直接，活性和还原染料染色的丝光和非丝光棉针织物的重量减少差异，可以检查预先存在的染料对纤维素酶活性的影响。本研究中的所有六种染料均抑制纤维素酶反应，抑制程度取决于染料类别。通过K / S估算，织物上染料的浓度是控制最终重量损失的最关键因素。与靛蓝染料相比分子大小更大的还原染料的平面结构似乎在其抑制作用中起着重要作用。与单反应性染料，直接染料和还原染料相比，双功能染料的抑制作用最大，这意味着双功能染料的交联特性与纤维素酶的水解有关。纤维素酶处理后的DMDHEU处理棉织物重量轻度减少，进一步支持了该建议。提出了一个模型来解释交联纤维素纤维上酶促裂解的缓慢过程。

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