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首页> 外文期刊>Textile Research Journal >Protein Soil Release Using Protease as Monitored with a Quartz Crystal Microbalance
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Protein Soil Release Using Protease as Monitored with a Quartz Crystal Microbalance

机译：使用蛋白酶和石英晶体微量天平监测蛋白质土壤释放

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摘要

Detergency is a complex procedure comprising both chemical and mechanical processes. In order to fully understand the chemical process, it is necessary to uncover its basic steps by setting up a simplified modeling system. In this study, we attempt to find the key to the detergency mechanism by revealing the adsorption of enzymes and surfactants onto proteins and the degradation and subsequent release of the proteins using a quartz crystal microbalance (QCM) measurement that can quantify minute weight changes in water over time. We first examine three different proteins-gelatin, ovalbumin(OvA), and keratin. The frequency change of a QCM is traced for the release of solid protein by Nagarse~® from the surface of a gold electrode. As observed in former studies of dissolved protein, the synergistic effects are confirmed for release by proteaseonionic surfactants, i.e., C_(12)(EO)_7 and C_(16)(EO)_7. Adding anionic surfactants-sodium dodecylbenzene-sulfonate (DBS) or sodium dodecylsulfonate (SDS)-enhances the release rate at near the critical micelle concentrations. The amount of adsorption of the enzyme to gelatin increases in the presence of anionic or nonionic surfactants.
机译：洗涤力是一个复杂的过程，包括化学过程和机械过程。为了完全理解化学过程，有必要通过建立简化的建模系统来揭示其基本步骤。在这项研究中，我们试图通过揭示酶和表面活性剂在蛋白质上的吸附以及通过使用可以量化水中微小重量变化的石英晶体微量天平（QCM）测量揭示蛋白质的降解和随后释放来发现去污机理的关键。随着时间的推移。我们首先检查三种不同的蛋白质-明胶，卵清蛋白（OvA）和角蛋白。 QCM的频率变化可追溯到Nagarse®从金电极表面释放出的固体蛋白。如先前对溶解蛋白的研究中所观察到的，通过蛋白酶/非离子表面活性剂即C_（12）（EO）_7和C_（16）（EO）_7的释放确认了协同作用。添加阴离子表面活性剂-十二烷基苯磺酸钠（DBS）或十二烷基磺酸钠（SDS）-可提高临界胶束浓度附近的释放速率。在阴离子或非离子表面活性剂的存在下，酶对明胶的吸附量增加。

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