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首页> 外文期刊>The Analyst >Discrimination of zone-specific spectral signatures in normal human prostate using Raman spectroscopy
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Discrimination of zone-specific spectral signatures in normal human prostate using Raman spectroscopy

机译：使用拉曼光谱法区分正常人前列腺中的区域特定光谱特征

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摘要

The prostate gland is the most common site of pathology in human males. Using the urethra as annanatomical reference point, it can be divided into three distinct zones known as the transition zone (TZ),nperipheral zone (PZ) and central zone (CZ). The pathological conditions of benign prostatic hypertrophynand/or prostate adenocarcinoma are highly prevalent in this gland. This preliminary study set out tondetermine whether biochemical intra-individual differences between normal prostate zones could benidentified using Raman spectroscopy with subsequent exploratory analyses. A normal (benign) prostatentransverse tissue section perpendicular to the rectal surface and above the verumontanumwas obtained inna paraffin-embedded block.A 10-mm-thick slice was floated onto a gold substrate, de-waxed and analysednusing Raman spectroscopy (200 epithelial-cell and 140 stromal spectra/zone). Raman spectra werensubsequently processed in the 1800–367 cmu00021 spectral region employing principal component analysisn(PCA) to determine whether wavenumber–intensity relationships expressed as single points in hyperspacenmight reveal biochemical differences associated with inter-zone pathological susceptibility. Visualisationnof PCA scores plots and their corresponding loadings plots highlighted 781 cmu00021 (cytosine/uracil) andn787 cmu00021 (DNA) as the key discriminating factors segregating PZ from less susceptible TZ and CZnepithelia (P < 0.001). Conversely, 1459 cmu00021 (lipids and proteins) and 1003 cmu00021 (phenylalanine) werenidentified as the key biochemical factor distinguishing TZ from CZ epithelia (P < 0.05). All stromal zonesnwere discriminated by the protein/lipid region (1459 cmu00021 and 1100 cmu00021) with DNA/RNA regionn(781 cmu00021 and 787 cmu00021) only highlighted between PZ and CZ (P < 0.05). This novel approach identifiesnbiochemicalmarkers that may have aetiological functional roles towards susceptibility of human prostatenzones to specific pathological conditions.
机译：前列腺是男性男性中最常见的病理部位。使用尿道作为解剖学参考点，可将其分为三个不同的区域，分别称为过渡区（TZ），周边区（PZ）和中心区（CZ）。良性前列腺肥大和/或前列腺腺癌的病理状况在该腺体中非常普遍。这项初步研究确定了正常前列腺区域之间的生化个体内差异是否可以通过拉曼光谱法和随后的探索性分析来鉴定。在石蜡包埋的块中获得垂直于直肠表面且垂直于疣膜的正常（良性）前列腺横断面，将10毫米厚的切片漂浮在金基底上，使用拉曼光谱仪（200个上皮细胞）进行脱蜡和分析和140层光谱/区域）。随后在1800-367 cmu00021光谱区域中使用主成分分析法（PCA）处理拉曼光谱，以确定在超高空中以单点表示的波数-强度关系是否显示出与区域间病理敏感性相关的生化差异。 PCA评分图的可视化及其对应的负荷图突出显示781 cmu00021（胞嘧啶/尿嘧啶）和n787 cmu00021（DNA）是将PZ与较不易感的TZ和CZnepithelia分离的关键因素（P <0.001）。相反，将1459 cmu00021（脂质和蛋白质）和1003 cmu00021（苯丙氨酸）确定为区分TZ和CZ上皮的关键生化因子（P <0.05）。通过蛋白质/脂质区域（1459 cmu00021和1100 cmu00021）和仅在PZ和CZ之间突出显示的DNA / RNA区域（781 cmu00021和787 cmu00021）来区分所有基质区域。这种新颖的方法鉴定了可能对人类前列腺素对特定病理状况易感性起病因学功能作用的生物化学标记。

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  • 来源
    《The Analyst》 |2010年第12期|p.3060-3069|共10页
  • 作者

    Imran I. Patel and Francis L. Martin;

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    Centre for Biophotonics, Lancaster Environment Centre, LancasterUniversity, Bailrigg, Lancaster, LA1 4YQ, UK. E-mail: f.martin@lancaster.ac.uk;

    Tel: +44 1524 510206† This article is part of a themed issue on Optical Diagnosis. This issueincludes work presented at SPEC 2010 Shedding Light on Disease:Optical Diagnosis for the New Millennium, which was held inManchester, UK June 26th–July 1st 2010.‡ Electronic supplementary information (ESI) available: PCA-LDA andparaffin-inclusive PCA analysis of inter-zone prostate differences. SeeDOI: 10.1039/c0an00518e;

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