Syrian hamster embryo (SHE) assay (pH 6.7) coupled with infrared spectroscopy and chemometrics towards toxicological assessment

Júlio Trevisan Plamen P. Angelov Imran I. Patel Ghazal M. Najand Karen T. Cheung Valon Llabjani Hubert M. Pollock Shannon W. Bruce Kamala Pant Paul L. Carmichael Andrew D. Scott and Francis L. Martin

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Syrian hamster embryo (SHE) assay (pH 6.7) coupled with infrared spectroscopy and chemometrics towards toxicological assessment

机译：叙利亚仓鼠胚胎（SHE）测定（pH 6.7）结合红外光谱和化学计量学进行毒理学评估

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The Syrian hamster embryo (SHE) assay (pH 6.7) is an in vitro candidate to replace in vivoncarcinogenicity tests. However, the conventional method of visual scoring of foci (non-transformed vs.ntransformed colonies) can be time-consuming and is open to subjectivity. Infrared (IR) spectroscopynhas the potential to provide objective assessment of such SHE colonies with the added advantage ofnpotentially providing mechanistic information. In this study, SHE cells were treated with one of eightndifferent chemical regimens, allowed in culture to attach and form foci on IR-reflective glass slides;nthese were subsequently interrogated by attenuated total reflection (ATR) Fourier-transform IRn(FTIR) spectroscopy. Derived mid-IR spectra (n ?13 406) were subjected to chemometric analysisnfocusing primarily on the extraction of biochemical information related to test agent treatment and/ornmorphological transformation. The use of ATR-FTIR spectroscopy with chemometrics to analyze thenSHE assay is a novel approach to toxicological assessment.

机译：叙利亚仓鼠胚胎（SHE）分析（pH 6.7）是替代体内致癌性测试的体外候选药物。但是，对病灶（未转化的菌落与未转化的菌落）进行视觉评分的常规方法可能很耗时，而且容易受到主观影响。红外（IR）光谱技术具有对此类SHE菌落进行客观评估的潜力，并且具有潜在地提供机械信息的附加优势。在这项研究中，SHE细胞采用了八种不同的化学方案之一进行处理，允许其在培养物中附着并在IR反射玻璃载玻片上形成焦点;然后通过衰减全反射（ATR）傅立叶变换IRn（FTIR）光谱对其进行询问。对衍生的中红外光谱（n = 13 406）进行化学计量分析，主要集中在提取与受试药物处理和形态学转变有关的生化信息。使用ATR-FTIR光谱和化学计量学分析SHE分析是一种新的毒理学评估方法。

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《The Analyst》 |2010年第12期|p.3266-3272|共7页
作者
Júlio Trevisan Plamen P. Angelov Imran I. Patel Ghazal M. Najand Karen T. Cheung Valon Llabjani Hubert M. Pollock Shannon W. Bruce Kamala Pant Paul L. Carmichael Andrew D. Scott and Francis L. Martin;
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aSchool of Computing and Communication Systems, Lancaster University,Lancaster, LA1 4WA, UKbCentre for Biophotonics, Lancaster Environment Centre, LancasterUniversity, Lancaster, LA1 4YQ, UK. E-mail: f.martin@lancaster.ac.uk;

Tel: +44 1524 510206cBioReliance Corporation, Medical Center Drive, Rockville, MD, 20850,USAdSafety and Environmental Assurance Centre, Unilever Colworth SciencePark, Bedfordshire, UKeDepartment of Physics, Lancaster University, Lancaster, LA1 4YB, UK† This article is part of a themed issue on Optical Diagnosis. This issueincludes work presented at SPEC 2010 Shedding Light on Disease:Optical Diagnosis for the New Millennium, which was held inManchester, UK June 26th–July 1st 2010.;

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1. Syrian hamster embryo (SHE) assay (pH 6.7) coupled with infrared spectroscopy and chemometrics towards toxicological assessment [J] . Trevisan J., Angelov P.P., Patel I.I., The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry . 2010,第12期

机译：叙利亚仓鼠胚胎（SHE）测定（pH 6.7）结合红外光谱和化学计量学进行毒理学评估
2. Classification of test agent-specific effects in the Syrian hamster embryo assay (pH 6.7) using infrared spectroscopy with computational analysis [J] . Abdullah A. Ahmadzai Júlio Trevisan Weiyi Pang Imran I. Patel Nigel J. Fullwood1 Shannon W. Bruce2 Kamala Pant2 Paul L. Carmichael3 Andrew D. Scott3 and Francis L. Martin* Mutagenesis . 2012,第3期

机译：使用计算机分析和红外光谱法对叙利亚仓鼠胚胎测定法（pH 6.7）中的测试剂特异性作用进行分类
3. Classification of test agent-specific effects in the Syrian hamster embryo assay (pH 6.7) using infrared spectroscopy with computational analysis [J] . AhmadzaiA.A., TrevisanJ., PangW., Mutagenesis . 2012,第3期

机译：使用计算机分析和红外光谱法对叙利亚仓鼠胚胎测定（pH 6.7）中测试剂的特定作用进行分类
4. Modulation of Transforming and Clastogenic Activities of Catechol Estrogens by a Catechol-O-methyltransferase Inhibitor in Syrian Hamster Embryo Fibroblasts [C] . Takeki Tsutsui, Takeo W. Tsutsui, Yukiko Tamura, International Symposium on Hormonal Carcinogenesis . 2005

机译：在叙利亚仓鼠胚胎成纤维细胞中的儿茶酚-O-甲基转移酶抑制剂的调制调节儿茶酚雌激素抑制剂
5. Prediction and Classification of Physical Properties by Near-Infrared Spectroscopy and Baseline Correction of Gas Chromatography Mass Spectrometry Data of Jet Fuels by Using Chemometric Algorithms. [D] . Xu, Zhanfeng. 2012

机译：使用化学计量学算法对喷气燃料的近红外光谱和物理校正进行气相色谱质谱数据的基线预测和分类。
6. A mechanistic evaluation of the Syrian hamster embryo cell transformation assay (pH 6.7) and molecular events leading to senescence bypass in SHE cells [O] . Jessica C. Pickles, Kamala Pant, Lisa A. Mcginty, -1

机译：机械评估叙利亚仓鼠胚胎细胞转化试验（pH 6.7）和导致SHE细胞衰老的分子事件
7. The Syrian hamster embryo (SHE) assay (pH 6.7): mechanisms of cell transformation and application of vibrational spectroscopy to objectively score endpoint alterations [O] . A. A. Ahmadzai, J. Trevisan, N. J. Fullwood, 2011

机译：叙利亚仓鼠胚胎（她）测定（pH 6.7）：细胞转化机制和振动光谱的应用，以客观地得分终点改变
8. Initial Submission: Letter from Procter & Gamble Co. to EPA Reporting Results from 7-Day Syrian Hamster Embryo (SHE) Transformation Assay of (Confidential), Dated 4/23/1999 (Sanitized) [R] . 1999

机译：初步提交：procter＆Gamble Co.致Epa报告结果，来自7天叙利亚仓鼠胚胎（sHE）转化分析（机密），日期为1999年4月23日（已消毒）

Syrian hamster embryo (SHE) assay (pH 6.7) coupled with infrared spectroscopy and chemometrics towards toxicological assessment

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