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首页> 外文期刊>The Analyst >Fourier transform infrared spectroscopic studies of T-cell lymphoma, B-cell lymphoid and myeloid leukaemia cell lines
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Fourier transform infrared spectroscopic studies of T-cell lymphoma, B-cell lymphoid and myeloid leukaemia cell lines

机译：T细胞淋巴瘤，B细胞淋巴样和髓样白血病细胞系的傅里叶变换红外光谱研究

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摘要

This paper presents Fourier transform infrared (FT-IR) spectroscopy to characterise spectralndifferences that distinguish cells derived from human T-cell lymphoma, B-cell lymphoid, and myeloidnleukaemia cell lines. This methodology is based on spectral measurements of major cellular biochemicalnconstituents and multivariate spectral processing. Major spectral differences were observed in the 1800–n900 cmu00011 ‘fingerprint’ spectral region. Bands in the averaged spectra for each cell line were assigned tonmajor biochemical constituents including: proteins, lipids, carbohydrates and nucleic acids.nMultivariate statistical analysis of the spectra was carried out to develop a classification model tondiscriminate the five cell types. The results show that FT-IR spectroscopy displays high sensitivity andnspecificity when discriminating between T-cell lymphoma, B-cell lymphoid, and myeloid leukaemiancells based on intrinsic biomolecular signatures. FT-IR spectroscopy in combination with multivariatenstatistical analysis provides an important insight into T-cell lymphoma, B-cell lymphoid, and myeloidnleukaemia cell line identification. In conclusion, this paper demonstrates a potential for this techniquento be used in developing a clinical tool for the detection and identification of haematologicalnmalignancies.
机译：本文提出了傅立叶变换红外（FT-IR）光谱技术，以表征能区分人类T细胞淋巴瘤，B细胞淋巴样和髓性白血病细胞株的细胞的光谱差异。该方法基于主要细胞生化成分的光谱测量和多元光谱处理。在1800–n900 cmu00011“指纹”光谱区域中观察到主要光谱差异。为每个细胞系的平均光谱中的谱带分配了主要的生化成分，包括：蛋白质，脂质，碳水化合物和核酸。n对光谱进行多变量统计分析以建立区分五种细胞类型的分类模型。结果表明，基于固有的生物分子特征，FT-IR光谱在区分T细胞淋巴瘤，B细胞淋巴瘤和髓样白血病细胞时显示出高灵敏度和特异性。 FT-IR光谱技术与多变量统计分析相结合，为T细胞淋巴瘤，B细胞淋巴瘤和骨髓性白血病细胞系鉴定提供了重要见识。总之，本文证明了该技术在开发用于检测和鉴定血液系统恶性肿瘤的临床工具中的潜力。

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    《The Analyst》 |2009年第4期|p.763-768|共6页
  • 作者

    Jaspreet Babrah Keith McCarthy Richard John Lush Adam David Rye Conrad Bessant and Nicholas Stone;

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    aBiophotonics Research Group, Gloucestershire Hospitals NHS FoundationTrust, Great Western Road, Gloucester, UK GL1 3NN. E-mail: j.babrah@medical-research-centre.com, n.stone@medical-research-centre.com, Fax:+44 (0)8454 225485, Tel: +44 (0)8454 225486bDepartment of Haematology, Gloucestershire Hospitals NHS FoundationTrust, Great Western Road, Gloucester, UK GL1 3NNcDepartment of Histopathology, Gloucestershire Hospitals NHSFoundation Trust, Great Western Road, Gloucester, UK GL1 3NNdCranfield Health, Cranfield University, CranfieldBedfordshire, UK MK430AL,;

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