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首页> 外文期刊>The Kasetsart Journal >Selection and Optimization for Lipid Production of a Newly Isolated Oleaginous Yeast, Rhodosporidium toruloides DMKU3-TK16
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Selection and Optimization for Lipid Production of a Newly Isolated Oleaginous Yeast, Rhodosporidium toruloides DMKU3-TK16

机译:一种新分离的产油酵母曲霉假单胞菌DMKU3-TK16脂质生产的选择和优化

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摘要

Rhodosporidium toruloides DMKU3-TK16, an oleaginous yeast, was isolated from soil by an enrichment technique in nitrogen-limited medium I. Lipid production by this strain was highest in shaking flask cultivation at 150 rpm and 28℃ in nitrogen-limited medium II containing per liter 70 g glucose, 0.75 g yeast extract, 0.55 g (NH_4)_2SO_4, 0.4 g KH_2PO_4, 2.0 g MgSO_4·7H_2O, with a pH of 5.5. Under these optimal conditions, when the C/N ratio of the medium was 140, R. toruloides DMKU3-TK16 produced a lipid quantity of 9.26 g/L, which was 71.30 % of the dry biomass (13.33 g/L) after 168 h of cultivation. The major fatty acids of the cellular lipid were oleic acid (41.54%), palmitic acid (22.49%), linoleic acid (15.12%) and steric acid (14.56%).
机译:在富氮条件下,通过富集技术从土壤中分离出油状酵母圆环假单胞菌DMKU3-TK16。在150 rpm和28℃含氮限制条件下,在28℃摇瓶中培养该菌株产生的脂质最高。升70克葡萄糖,0.75克酵母提取物,0.55克(NH_4)_2SO_4、0.4克KH_2PO_4、2.0克MgSO_4·7H_2O,pH值为5.5。在这些最佳条件下,当培养基的C / N比为140时,拟南芥DMKU3-TK16产生的脂质量为9.26 g / L,在168 h后占干生物量(13.33 g / L)的71.30%。种植。细胞脂质的主要脂肪酸为油酸(41.54%),棕榈酸(22.49%),亚油酸(15.12%)和立体酸(14.56%)。

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