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首页> 外文期刊>The Kasetsart Journal >Effects of Extender, Cryoprotectant and Freezing Protocols on Post-thaw Sperm Motility, Morphology and Viability of Three-yellow Cocks (Gallus domesticus) Spermatozoa
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Effects of Extender, Cryoprotectant and Freezing Protocols on Post-thaw Sperm Motility, Morphology and Viability of Three-yellow Cocks (Gallus domesticus) Spermatozoa

机译：补充剂，低温保护剂和冷冻方案对解冻后三只黄色家鸡精子运动能力，形态和活力的影响

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摘要

Semen samples collected from 10 three-yellow cocks (Gallus domesticus) were pooled and treated to determine the effect of extender and cryoprotectant (experiment 1) and the effect of freezing protocol and cryoprotectant (experiment 2) on frozen/thawed semen quality. In the first experiment, semen samples were diluted 1:1 (v:v) with two different semen extenders, Beltsville poultry semen extender (BPSE) or modified Tyrode's medium (TALP) and cryopreserved with either 6% dimethyl acetamide (DMA) or 8% dimethyl sulfoxide (DMSO). The best result (p<0.05) was obtained for TALP extender + 8% DMSO, with values of 57.32% for viability, 56.57% for normal morphology and 48.50% for motility. In experiment 2, two different freezing protocols (one-step and two-step) were evaluated. The results showed that there was no difference in post-thaw motility and viability of the spermatozoa (sperm) when either the one-step or the two-step protocol was applied with 8% DMSO. In contrast, the sperm motility and viability with the one-step protocol were better (p<0.05) than those with the two-step protocol when 6% DMA was used. This study indicated that from in vitro analysis, TALP with 8% DMSO was the preferred freezing solution for three-yellow cock sperm and this could be carried out using a one-step freezing protocol.
机译：收集并处理从10只三黄公鸡（Gallus domesticus）收集的精液样品，以确定增量剂和防冻剂的作用（实验1）以及冷冻方案和防冻剂的作用（实验2）对冷冻/解冻精液质量的影响。在第一个实验中，精液样品用两种不同的精液增量剂（贝尔茨维尔家禽精液增量剂（BPSE）或改良的Tyrode培养基（TALP））以1：1（v：v）稀释，并冷冻保存有6％二甲基乙酰胺（DMA）或8 ％的二甲基亚砜（DMSO）。对于TALP补充剂+ 8％DMSO，获得了最佳结果（p <0.05），活力的值分别为57.32％，正常形态的56.57％和运动力的48.50％。在实验2中，评估了两种不同的冷冻方案（一步和两步）。结果表明，当单步或两步方案与8％DMSO配合使用时，精子的解冻后活力和活力无差异。相比之下，当使用6％DMA时，单步操作的精子活力和生存力要好于两步操作的精子活力和生存力（p <0.05）。这项研究表明，从体外分析来看，含8％DMSO的TALP是三黄公鸡精子的首选冷冻溶液，可以使用一步冷冻方法进行冷冻。

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  • 来源
    《The Kasetsart Journal》 |2009年第2期|269-279|共11页
  • 作者

    Wirasak Fungfuang; Voravit Siripholvat; Suchart Sa-nguanphan; Anuchai Pinyopummin;

  • 作者单位

    Center of Agricultural Biotechnology, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom 73140, Thailand;

    Department of Animal Science, Faculty of Agriculture at Kamphaeng Sean, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom 73140, Thailand;

    Department of Animal Science, Faculty of Agriculture at Kamphaeng Sean, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom 73140, Thailand;

    Department of Large Animal and Wildlife Clinical Science, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom 73140, Thailand;

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  • 正文语种 eng
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  • 关键词

    three-yellow chicken; gallus domesticus; extender; cryoprotectant; frozen semen;

    机译：三黄鸡家鸡补充剂防冻剂;冷冻精液;

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