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首页> 外文期刊>The Kasetsart Journal >Characterization Of The Antibacterial Activity And Probiotic Properties Of Lactic Acid Bacteria Isolated From Raw Fishand Nham-plaa
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Characterization Of The Antibacterial Activity And Probiotic Properties Of Lactic Acid Bacteria Isolated From Raw Fishand Nham-plaa

机译：生鱼和Nham-plaa中乳酸菌的抗菌活性和益生菌性质的表征

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摘要

Isolates of lactic acid bacteria from fish fillets and nham-plaa were screened to select the most suitable strains for the formulation of starter cultures with probiotic properties. Using an agar spot test, 30 of the 180 isolates that were tested inhibited at least one of four indicator strains; Staphylococcus aureus TISTR 118, Pediococcus acidilactici TISTR 051, Lactobacillus bulgaricus TISTR 415 and Listeria monocytogenes DMST 11256. Two bacterial isolates, 13IS3 and 13IS4, from fish fillets were resistant to a low pH in lactic acid (pH 2.9-3.2) and in HC1 (pH 2.2) and a high concentration of NaCl (6.0%) and bile salts (5.25%). These isolates were identified by morphological, biochemical and molecular analysis as Lactococcus lactis 13IS3 and Lactobacillus sakei 13IS4. The fermentation of nham-plaa model broth (NMB) by L. lactis 13IS3 seemed to be stimulated by 1.0% garlic extract and 0-1.5% NaCl at all temperatures of incubation. L. sakei 13IS4 fermented at a slower rate than L. lactis 13IS3, but fermented more rapidly in a 3.0%-garlic extract NMB with 0-1.5% NaCl at 30℃ and in the same medium containing 1.0-1.5% NaCl at 35℃.
机译：从鱼片和nham-plaa中分离出乳酸菌，以选择最合适的菌株来配制具有益生菌特性的发酵剂。使用琼脂斑点测试，被测的180种分离物中有30种抑制了四种指示菌株中的至少一种；金黄色葡萄球菌TISTR 118，乳酸双球菌TISTR 051，保加利亚乳杆菌TISTR 415和单核细胞增生李斯特菌DMST11256。鱼片的两种细菌分离物13IS3和13IS4对乳酸（pH 2.9-3.2）和HC1（ pH 2.2）和高浓度的NaCl（6.0％）和胆盐（5.25％）。通过形态，生化和分子分析将这些分离物鉴定为乳酸乳球菌13IS3和清酒乳杆菌13IS4。在所有温育温度下，乳酸乳球菌13IS3对nham-plaa模型肉汤（NMB）的发酵似乎受到1.0％大蒜提取物和0-1.5％NaCl的刺激。日本清酒乳杆菌13IS4的发酵速度要比乳酸乳杆菌13IS3慢，但在30°C的含0-1.5％NaCl的3.0％大蒜提取物NMB中和在35°C的含1.0-1.5％NaCl的相同培养基中发酵更快。

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