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首页> 外文期刊>The Kasetsart Journal >Extracellular Halophilic Ribonuclease from a Halotolerant Pseudomonas sp.: Purification and Characterization
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Extracellular Halophilic Ribonuclease from a Halotolerant Pseudomonas sp.: Purification and Characterization

机译：耐盐假单胞菌属的细胞外嗜盐核糖核酸酶：纯化和表征。

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A halotolerant bacterium, Pseudomonas sp. No. 3241, isolated from Thai fish sauce obtained in Suratthani province, produced an extracellular ribonuclease when cultivated aerobically without NaCl in Sehgal and Gibbons complex medium (SGC medium). Ribonuclease was purified by ethanol precipitation, Sephadex G-150 gel filtration and DEAEToyopearl 650M anion-exchange chromatography. The purity and molecular weight were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The result showed that the molecular weight of ribonuclease from halotolerant Pseudomonas sp. No. 3241 was 61,000 daltons. Purified ribonuclease was optimum at pH 10.0 and at temperature of 40℃. Purified ribonuclease was stable between pH 6.0 and 10.0 and at temperatures between 30 and 40℃. This enzyme had marked halophilic enzyme properties that required an optimum NaCl concentration of 3.0 M (18%).
机译：耐盐细菌，Pseudomonas sp.。从Suratthani省获得的泰国鱼露中分离出的No.3241，在Sehgal和Gibbons复合培养基（SGC培养基）中，在无NaCl需氧条件下培养时，产生了细胞外核糖核酸酶。核糖核酸酶通过乙醇沉淀，Sephadex G-150凝胶过滤和DEAEToyopearl 650M阴离子交换色谱纯化。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）确定纯度和分子量。结果显示，来自卤代假单胞菌假单胞菌sp。的核糖核酸酶的分子量。 3241是61,000道尔顿。纯化的核糖核酸酶的最佳pH值为10.0，温度为40℃。纯化的核糖核酸酶在pH 6.0至10.0以及30至40℃的温度下均稳定。该酶具有显着的嗜盐酶特性，需要3.0 M（18％）的最佳NaCl浓度。

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