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首页> 外文期刊>The Kasetsart Journal >Identification of Major Saponins from Jiaogulan Extract (Gynostemma pentaphyllum)
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Identification of Major Saponins from Jiaogulan Extract (Gynostemma pentaphyllum)

机译：绞股蓝提取物（绞股蓝）中主要皂苷的鉴定

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摘要

Jiaogulan (Gynostemma pentaphyllum; GP) contained a saponin glycoside which was related to ginseng saponin. The purpose of this study was to identify the main saponin in water extract GP compared to methanol and ethanol extract. The sample was prepared by alkaline cleavage, trimethylsilyl (TMS) derivatization and gas chromatographic-mass spectrometric (GC-MS) analysis comparison with standard ginsenoside Rb1. The results showed that there were two main dammarant type aglycones in water, methanol and ethanol extracts GP, 20(S)-dammar-24-ene-3β, 12β, 20 triol and 20(S)-dammar-24-ene-3β, 6α, 12β, 20 tetrol, which were the same compound as ginsenoside Rb1 and Rg1 from ginseng (Panax spp.), representatively. The other peak, 20(S)-dammar-24-ene-3β, 12β, 19, 20 tetrol, was not obvious in water extract as in methanol and ethanol extracts. In conclusion, the water extract GP contained two main saponin compounds, which were the same compounds as in ginseng.
机译：绞股蓝（Gynostemma pentaphyllum； GP）含有与人参皂苷有关的皂苷苷。本研究的目的是鉴定与甲醇和乙醇提取物相比，水提取物GP中的主要皂苷。通过碱裂解，三甲基甲硅烷基（TMS）衍生化和气相色谱-质谱（GC-MS）分析与人参皂苷Rb1的标准品制备样品。结果表明，水，甲醇和乙醇提取物中的GP，20（S）-dammar-24-ene-3β，12β，20三醇和20（S）-dammar-24-ene-3β有两种主要的dammarant型糖苷配基。 ，6α，12β，20萜醇，分别与人参中的人参皂甙Rb1和Rg1（Panax spp。）相同。在水提取物中，另一个峰20（S）-dammar-24-ene-3β，12β，19、20的四氢噻吩不明显，在甲醇和乙醇提取物中。总之，水提取物GP含有两种主要的皂苷化合物，与人参中的化合物相同。

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