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首页> 外文期刊>The Kasetsart Journal >Attempt to Practical Use of Lactobacillus plantarum SP 1-3 in Spray Dried Granule Form for Making Good Quality Silage in Thailand
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Attempt to Practical Use of Lactobacillus plantarum SP 1-3 in Spray Dried Granule Form for Making Good Quality Silage in Thailand

机译：尝试以喷雾干燥颗粒形式实际使用植物乳杆菌SP 1-3在泰国生产优质青贮饲料

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摘要

For the practical application of spray-dried granule of Lactobacillus plantarum SP 1-3 isolated from a corn silage prepared in Thailand, a low-cost and convenient culture medium formulated from available materials in Thailand was designed for the large scale culture. The best formulation of culture medium was 2% molasses, 0.5% rice bran, 0.2% yeast extract, 0.2% Na-acetate, 0.05% KH_2PO_4, 0.02% MgSO_4 and 0.01% MnSO_4. 4H_2O and was used for cultivating spray-dried granule strain SP 1-3. This SP 1-3 granule was stable and kept as the living cell count of 10~(8～9) cfu/g in storing at 4℃ for 28 days and surely improved the fermentation quality of Napier grass silage. To use this granule for the preparation of practical scale silage, the low-cost and convenient method to increase the cell mass of Lactic acid bacteria (LAB) strain was developed by using a 5-liter plastic bottled drinking water. The culture solution of SP 1-3 obtained by this method was named "PBS-SP". The fermentation qualities of Pangola grass and Erianthus silages inoculated with PBS-SP were improved effectively. However, the inoculation of LAB strain has no effect on the reduction of living yeast cell in silage and aerobic deterioration of silage by yeast was remained as a problem to be solved.
机译：为了从泰国制备的玉米青贮饲料中分离出植物乳杆菌SP 1-3喷雾干燥颗粒的实际应用，设计了一种由泰国可用材料配制的低成本便捷培养基，用于大规模培养。最好的培养基配方是2％糖蜜，0.5％米糠，0.2％酵母提取物，0.2％乙酸钠，0.05％KH_2PO_4、0.02％MgSO_4和0.01％MnSO_4。 4H_2O，用于培养喷雾干燥的颗粒菌株SP 1-3。该SP 1-3颗粒稳定，在4℃下保存28天，活细胞数为10〜（8〜9）cfu / g，可以肯定地提高了纳皮尔青贮饲料的发酵质量。为了将这种颗粒用于制备实用规模的青贮饲料，通过使用5升塑料瓶装饮用水开发了一种低成本，方便的方法来增加乳酸菌（LAB）菌株的细胞质量。通过该方法获得的SP 1-3的培养液称为“ PBS-SP”。接种PBS-SP可以有效提高Pangola草和Erianthus青贮饲料的发酵质量。然而，LAB菌株的接种对青贮饲料中的活酵母细胞的减少没有影响，并且仍然存在待解决的问题，即酵母对青贮饲料的有氧降解。

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