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首页> 外文期刊>The Kasetsart Journal >Molecular Identification of Encephalartos (Zamiaceae) Species and Their Relationships to Morphological Characters
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Molecular Identification of Encephalartos (Zamiaceae) Species and Their Relationships to Morphological Characters

机译：脑病（Zamiaceae）种类的分子鉴定及其与形态特征的关系

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摘要

RAPD and morphological characteristics were used to evaluate genetic similarity among twenty species of Encephalartos geographically distributed in several African countries. Eight out of sixty primers, i.e. OPA-05, OPA-07, OPB-07, OPB-08, OPB-11, OPJ-07, OPJ-13, and OPJ-15 could produce polymorphic and specific band patterns to identify these cycads. The primers generated 240 reproducible bands at the size of 0.15 - 2.20 kb. Polymorphism of the DNA profiles appraised by NTSYS program gave the similarity coefficient in the range of 0.53-0.80. Phylogenetic tree was constructed and twenty Encephalartos species were grouped into six clusters. The grouping corresponded well to the nine morphological characters (stem, color of rachis, leaf curvation, spiny leaflet, number of prickle, leaflet shape, keel, upper and lower margins of leaflet) as well as to their original geographic distribution.
机译：RAPD和形态学特征被用于评估地理分布在几个非洲国家的20种Encephalartos的遗传相似性。六十个引物中的八个，即OPA-05，OPA-07，OPB-07，OPB-08，OPB-11，OPJ-07，OPJ-13和OPJ-15可以产生多态性和特异性条带模式来识别这些苏铁。引物产生了240条可复制的条带，大小为0.15-2.20 kb。通过NTSYS程序评估的DNA图谱的多态性给出了0.53-0.80范围内的相似系数。建立了系统发育树，并将二十种脑脑动物分为六个簇。该分组与九个形态特征（茎，叶片的颜色，弯曲的叶片，多刺的小叶，刺的数量，小叶的形状，龙骨，小叶的上边缘和下边缘）以及它们的原始地理分布非常吻合。

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