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首页> 外文期刊>The Kasetsart Journal >Production and Partial Characterization of Chitosanases from a Newly Isolated Bacillus cereus
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Production and Partial Characterization of Chitosanases from a Newly Isolated Bacillus cereus

机译：新分离的蜡状芽孢杆菌壳聚糖酶的生产和部分表征

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摘要

The production of chitosanases by a newly isolated Bacillus cereus TP12.24 was studied both in shake flask and fermenter cultures. The M9-chitosan medium was found most suitable with 0.5% chitosan as a sole carbon source optimized under aerobic growth conditions at pH 6.0 and 30℃. The specific rates of growth, substrate consumption, and enzyme production were improved using controlled completely aerobic conditions in 2-1 fermenter. While the yield of biomass was considerably increased, the enzyme yield was on the contrary decreased. As a result, the volumetric chitosanases productivity was 43.55 U/l h, which was 1.2 times that obtained from shake flask culture due to higher specific rates of chitosan consumption and chitosanases production. In this work, the crude chitosanases from Bacillus cereus TP12.24 showed their optimal pH and temperature at 6.5 and 55℃, while the stabilities to pH and temperature were found at 3.0-8.0 and 30-50℃, respectively. The Bacillus cereus chitosanases could be used for preparing the chitosano-oligosaccharides under mild temperature.
机译：在摇瓶和发酵罐中研究了新分离的蜡状芽孢杆菌TP12.24产生的壳聚糖酶。发现M9-壳聚糖培养基最适合以0.5％的壳聚糖作为唯一碳源，并在pH 6.0和30℃的有氧生长条件下进行了优化。在2-1发酵罐中，通过控制完全有氧条件，可以提高特定的生长速率，底物消耗和酶的产生。尽管生物量的产率大大提高，但酶的产率却相反地降低。结果，由于较高的脱乙酰壳多糖消耗率和脱乙酰壳多糖酶的生产速率，脱乙酰壳多糖的生产率为43.55U / l·h，是摇瓶培养的1.2倍。在这项工作中，蜡状芽孢杆菌TP12.24的粗壳聚糖酶在6.5和55℃时显示最佳的pH和温度，而对pH和温度的稳定性分别在3.0-8.0和30-50℃。蜡状芽孢杆菌的壳聚糖酶可用于在温和的温度下制备壳聚糖寡糖。

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