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首页> 外文期刊>The Kasetsart Journal >Combining Ability of Inbred Lines Derived from Quality Protein Maize Populations
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Combining Ability of Inbred Lines Derived from Quality Protein Maize Populations

机译：优质蛋白玉米种群自交系的配合力

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Quality protein maize (QPM) with improved kernel quality over O_2O_2 soft genotypes was developed by introducing modifier genes and selecting for a hard, vitreous endosperm in O_2O_2 germplasm at the International Maize and Wheat Research Center (CIMMYT). Three QPM populations developed by CIMMYT were used in the research. S_0-plants of preferred morphological characters were self-pollinated to produce S_1 until S_3 lines. Ten S_3 inbred lines were examined for the opaque-2 gene using the phi057 marker and a diallel cross was made. The experiment was conducted in a 7 x 7 simple lattice design at the National Corn and Sorghum Research Center, Thailand. The results showed that 10 inbred lines were detected with polymorphism the same as opaque-2 maize (O_2O_2) but were different from non-opaque-2 maize. The protein content in endosperm of these inbred lines ranged from 7.76 to 8.61% while those of opaque-2 and non-opaque-2 maize contained about 8.45 and 8.73%, respectively. However, the protein content of inbred lines, the diallel cross and check variety were not significantly different. Means of grain yield (t ha~(-1)) were 4.48 for the diallel cross (F_1), 1.97 for inbred lines, 3.30 for opaque-2 and 6.61 for non-opaque-2 hybrid check. However, grain yield of the best diallel cross was 6.10 t ha~(-1) which was not significantly different from the non-opaque-2 hybrid. Protein contents were not significant among the diallel cross, opaque-2 and non- opaque-2 hybrids. Tryptophan content in endosperm of the diallel cross was higher than non-opaque-2 hybrids. Inbred P10 had the best combination of GCA effects for grain yield. Moreover, inbred lines, P1, P7, P8 and P9 also gave positive GCA effects.
机译：通过在国际玉米和小麦研究中心（CIMMYT）引入修饰基因并在O_2O_2种质中选择坚硬的玻璃质胚乳，开发了具有比O_2O_2软基因型更高的籽粒品质的优质蛋白玉米（QPM）。该研究使用了CIMMYT开发的三个QPM种群。具有最佳形态特征的S_0植物自花授粉以产生S_1直至S_3品系。使用phi057标记检查了10个S_3自交系的opaque-2基因，并进行了Dialelel杂交。该实验是在泰国国家玉米和高粱研究中心以7 x 7简单晶格设计进行的。结果表明，共检测到10个自交系，其多态性与不透明2号玉米（O_2O_2）相同，但与非不透明2号玉米不同。这些自交系的胚乳中蛋白质含量范围为7.76％至8.61％，而不透明2和非不透明2玉米的蛋白质含量分别约为8.45和8.73％。然而，自交系，二代杂交和对照品种的蛋白质含量没有显着差异。二叉杂交（F_1）的籽粒平均产量（t ha〜（-1））为4.48，自交系的籽粒平均产量为（t ha〜（-1）），不透明2的平均产量为3.30，不透明2的杂交检查为6.61。然而，最佳二叉杂交的产量为6.10 t ha〜（-1），与非不透明2杂种无显着差异。在二叉杂交，不透明2和非不透明2杂种中，蛋白质含量不显着。胚乳十字架胚乳中的色氨酸含量高于非不透明2杂种。自交P10对谷粒产量具有最佳的GCA作用组合。此外，自交系P1，P7，P8和P9也具有积极的GCA效应。

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