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首页> 外文期刊>The Kasetsart Journal >Biochemical Properties of Nile Tilapia (Oreochromis niloticus) emoglobin
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Biochemical Properties of Nile Tilapia (Oreochromis niloticus) emoglobin

机译：尼罗罗非鱼血红蛋白的生化特性

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摘要

Hemoglobins of O. niloticus (averaged standard length of 18.07 ± 0.89 cm and weight of 216.86 ± 27.13 g) were analysed of some biochemical properties. Average hematocrits were 25.80 ± 3.71 % and average hemoglobin concentrations were 7.05 ± 1.08 g/dl. It was found that there were 9-11 types of hemoglobin by cellulose acetate electrophoresis. The anodic and cathodic hemoglobins were revealed by native polyacrylamide gel electrophoresis with approximate amounts of 45.72 and 54.28% of total hemoglobin, respectively. In addition, the cathodic hemoglobin was classified by cellulose acetate electrophoresis as cathodic Ⅰ and cathodic Ⅱ with approximate amounts of 47.62 and 6.66% of total hemoglobin, respectively. Mass spectrometry was performed to determine molecular weights of hemoglobin. It was found that the molecular weights of native hemoglobin was 63.0 kDa and the molecular weights of subunits were 15.3, 15.6 and 16.1 kDa. The pis of anodic and cathodic hemoglobins ranged 5.28 - 6.80 and 7.27 - 9.71 , respectively.
机译：分析了尼罗罗非鱼的血红蛋白（平均标准长度为18.07±0.89 cm，重量为216.86±27.13 g）的一些生化特性。平均血细胞比容为25.80±3.71％，平均血红蛋白浓度为7.05±1.08 g / dl。通过乙酸纤维素电泳发现有9-11种血红蛋白。阳极和阴极血红蛋白通过天然聚丙烯酰胺凝胶电泳显示，分别占总血红蛋白的约45.72和54.28％。另外，通过乙酸纤维素电泳将阴极血红蛋白分类为阴极Ⅰ和阴极Ⅱ，分别占总血红蛋白的47.62和6.66％。进行质谱分析以确定血红蛋白的分子量。发现天然血红蛋白的分子量为63.0kDa，亚基的分子量为15.3、15.6和16.1kDa。阳极和阴极血红蛋白的pis分别为5.28-6.80和7.27-9.71。

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