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首页> 外文期刊>The Kasetsart Journal >Isolation of Drug Resistant and Enterotoxin Producing Staphylococcus aureus from Cafeterial Foods in Hospitals in Bangkok
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Isolation of Drug Resistant and Enterotoxin Producing Staphylococcus aureus from Cafeterial Foods in Hospitals in Bangkok

机译：从曼谷医院的咖啡馆食品中分离出抗药性和产生肠毒素的金黄色葡萄球菌

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The 525 food samples were examined for enterotoxin producing and drug resistance strains of Staphylococcus aureus. The 277 food samples from 12 government hospitals and 248 food samples from 12 private hospitals were examined. The 132 isolates of Staphylococcus aureus were found from 53(19.13%) food samples of government hospitals and also the 100 isolates were found from 41 (16.53%) food samples of private hospitals. Two hundred and twenty six out of 232 isolates of Staphylococcus aureus were coagulase positive and 74 out of 226 coagulase positive strains could produce enterotoxin. The types of enterotoxin produced by these 74 isolates were A,B,C,A&B and A&C of 13,28,23,4 and 6 isolates, respectively. The detection for drug resistance strains of 74 isolates that produced enterotoxin was doned by agar disc diffusion of Kirby-Bauer sensitivity test. The eight kinds of antibiotics and sulfonamides were selected base on the machanism of drugs, they were oxacillin, ciprofloxacin, rifampicin, vancomycin, gentamicin, tetracycline, chloramphenicol and co-trimoxazole. It was found that all isolates were sensitive to oxacillin,ciprofloxacin and rifampin (100%). Some were resisted to tetracycline (43.24%), chloramphenicol (17.57%) and gentamicin (4.05%), a few were intermediate to chloramphenicol, vancomycin and co-trimoxazole (trimethoprim/sulfamethoxazole). The multidrug-resistance strains were not found in this study.
机译：检查了525个食物样品中产生肠毒素和金黄色葡萄球菌的耐药菌株。检查了12家政府医院的277个食品样本和12家私立医院的248个食品样本。从公立医院的53份食品样本中发现了132株金黄色葡萄球菌，从私立医院的41份食品样本中发现了100株（16.53％）。 232株金黄色葡萄球菌中有262株为凝固酶阳性，而226株凝固酶阳性菌株中有74株产生肠毒素。这74个分离株产生的肠毒素类型分别为13、28、23、4和6个分离株的A，B，C，A＆B和A＆C。 74个产生肠毒素的菌株的耐药菌株的检测是通过Kirby-Bauer敏感性试验的琼脂圆盘扩散进行的。根据药物机理选择了八种抗生素和磺胺类药物，分别为奥沙西林，环丙沙星，利福平，万古霉素，庆大霉素，四环素，氯霉素和复方新诺明。发现所有分离株均对奥沙西林，环丙沙星和利福平敏感（100％）。一些对四环素（43.24％），氯霉素（17.57％）和庆大霉素（4.05％）具有抗性，少数对氯霉素，万古霉素和复方新诺明（甲氧苄氨嘧啶/磺胺甲恶唑）具有抗性。在这项研究中未发现多药耐药菌株。

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