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首页> 外文期刊>The Kasetsart Journal >A Study on Seed Germination and Seedling Development of Spathoglottis Bl. Orchids
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A Study on Seed Germination and Seedling Development of Spathoglottis Bl. Orchids

机译：tho草种子的萌发和幼苗发育的研究。兰花花

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The experiments were conducted to study characteristics of seed from self-pollination, intraspecific and interspecific crosses, seed germination and seedling growth of S. plicata and S. kimballiana in vitro. Seeds of S. plicata from capsule at 15,20,25 and 30 days and of S. kimballiana at 30, 35 and 40 days of age after pollination were germinated in modified VW solid and liquid media in vitro. The results showed that immature seeds in 15-day-old capsules of S. plicata had the lowest percentage of fertile seeds, germination rate and germination index. Self-pollinated seeds of S. kimballiana had lower percentage of fertile seeds in 30-day-old capsule while there was no difference in either intraspecific and interspecifics crosses. Seeds in liquid medium showed faster and better germination percentage than that on solid medium. After in vitro germination at 33±2℃ and 3,000 lux of light intensity, seeds were swollen and showed green embryos in liquid medium in 17-22 days which was 6 days faster than in solid medium. The germination rate and index were observed one month after sowing the seeds. There was no germination in 15-day-old capsule of all crosses of S. plicata. The 25- and 30-day-old capsules of S. plicata and 30-, 35- and 40-day-old capsules of S. kimballiana showed nigh germination rate and germination index. Seeds in liquid medium developed better than those in solid medium. After one month from germination, seeds in 15 day capsule of S. plicata germinated and developed to large-sized seedlings within 4 months. The 0, 0.01, 0.1 and 1 mg/l paclobutrazol had no significant effects on seedling growth of interspecifics cross (S. kimballiana x S. plicata) while 100 g/l blended banana in modified VW medium promoted seedling growth in fresh weight, plant height, leaf number, leaf length and root number.
机译：进行了实验，以研究自花授粉，种内和种间杂交，S。plicata和S. kimballiana的种子自发授粉，种子萌发和幼苗生长。授粉后15、20、25和30天来自荚膜的S. plicata种子和授粉后30、35和40天分别对Kimballiana的种子在改良的VW固体和液体培养基中萌发。结果表明，在15天大的S. plicata胶囊中，未成熟种子的可育种子百分率，发芽率和发芽指数最低。自花授粉的S. kimballiana种子在30天大的胶囊中可育种子的百分比较低，而种内和种间杂交均无差异。液体培养基上的种子比固体培养基上的种子发芽率更快，更好。在33±2℃和3000 lux的光强度下体外萌发后，种子在液体培养基中溶胀并在17-22天后显示绿色胚芽，比固态培养基快6天。播种1个月后观察发芽率和发芽指数。在15天大的囊状链球菌杂交中没有发芽。 25和30天大的链球菌胶囊以及30、35和40天的金链球菌胶囊显示了较高的发芽率和发芽指数。液体培养基中的种子发育好于固体培养基中的种子。发芽一个月后，在15天囊状链球菌种子中发芽，并在4个月内发育成大型幼苗。 0、0.01、0.1和1 mg / l多效唑对种间杂交（S. kimballiana x S. plicata）幼苗的生长没有显着影响，而在改良大众培养基中100 g / l混合香蕉可促进鲜重，植物的幼苗生长。高度，叶数，叶长和根数。

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