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首页> 外文期刊>The Kasetsart Journal >Hematology, Morphology, Cytochemistry and Ultrastructure of Blood Cells in Painted Stork (Mycteria leucocephala)
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Hematology, Morphology, Cytochemistry and Ultrastructure of Blood Cells in Painted Stork (Mycteria leucocephala)

机译：白鹳（Mycteria leucocephala）血细胞的血液学，形态学，细胞化学和超微结构

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摘要

Blood samples of 12 (7 males and 5 females) painted storks (Mycteria leucocephala), at the Bangpra Waterbird Breeding and Research Center, were collected from the cutaneus ulna vein for basic hematologic, light microscopic and transmission electron microscopic studies of blood cells. There was no hematozoa detected in all painted storks. There was no significant difference in all hematological values between the male and female groups. Lymphocytes were the most commonly observed leukocytes in the male painted storks and average 6-8 μm in diameter. Heterophils were the most commonly observed leukocytes in the females and were the second most commonly observed leukocytes in the males. Heterophils contained many pleomorphic, dull eosinophilic granules, ribosomes and lobed nucleus. Heterophils were the medium-sized cells, average 8-12 μm in diameter. Heterophils stained strongly positive with α-naphthyl acetate esterase (ANAE). Eosinophils revealed many small round granules and usually were the largest leukocytes, average 9-14 μm in diameter. The number of eosinophils was quite high in both the male and female painted storks. Eosinophil granules stained intensely with β-glucuronidase (β-glu), moderately to strong stained with ANAE and also stained with new methylene blue. Basophils were as small as lymphocytes but revealed many small granules surrounded central round nucleus. Basophils stained moderately with β-glu. Transmission electron microscopic examination revealed organelles within erythrocytes, heterophils, eosinophils and basophils.
机译：在班普拉水鸟繁殖和研究中心，从尺cut静脉收集了12只（7只雄性和5只雌性）彩斑鹳（Mycteria leucocephala）的血样，用于血液的基本血液学，光学显微镜和透射电子显微镜研究。在所有涂漆的鹳中均未检测到血红素。男性和女性组之间所有血液学值均无显着差异。淋巴细胞是雄性涂漆鹳中最常见的白细胞，平均直径为6-8μm。异嗜细胞是女性中最常见的白细胞，是男性中第二常见的白细胞。异嗜性细胞含有许多多形的，嗜酸性的嗜酸性颗粒，核糖体和浅裂的核。嗜异性细胞是中等大小的细胞，平均直径为8-12μm。嗜异性细胞被α-萘乙酸乙酸酯酶（ANAE）染色为强阳性。嗜酸性粒细胞显示出许多小的圆形颗粒，通常是最大的白细胞，平均直径为9-14μm。雌雄彩绘鹳的嗜酸性粒细胞数量都很高。嗜酸性粒细胞被β-葡萄糖醛酸苷酶（β-glu）强烈染色，中度到强度被ANAE染色，还被新的亚甲基蓝染色。嗜碱性粒细胞与淋巴细胞一样小，但在中央圆核周围有许多小颗粒。嗜碱性粒细胞用β-glu中度染色。透射电子显微镜检查显示红细胞，异嗜性，嗜酸性粒细胞和嗜碱性粒细胞内的细胞器。

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