Direct Shoot Bud Formation and Plantlet Regeneration form Culturing Leaf Discs of Neem (Azadirachta excelsa L.)

Sompong Tachatoe; Osnuma Rurgnoi

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Direct Shoot Bud Formation and Plantlet Regeneration form Culturing Leaf Discs of Neem (Azadirachta excelsa L.)

机译：印Ne叶盘的直接芽芽形成和小植株再生培养（印za（Azadirachta excelsa L.）

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Direct shoot bud formation and plantlet regeneration was induced from leaf discs of neem (Azadirachta excelsa (Jack) Jacobs) at various ages of development. The cultures were carries out in various kinds of media supplemented with various kinds and concentrations of plant growth regulators. Excised single shoots of more than 10 mm in length were rooted by different methods. The results revealed that leafdiscs from leaf at the age of 10-14 days after flushing gave shoot buds from 31 to 37 buds/disc on Murashige and Skoog (MS) medium containing 10 μM α-naphthaleneacetic acid (NAA) and 1 μm kinetin (KN).

机译：在不同发育时期，从印em叶盘（Azadirachta excelsa（Jack）Jacobs）诱导直接的芽芽形成和小植株再生。培养在各种培养基中进行，辅以各种种类和浓度的植物生长调节剂。切出的长度超过10 mm的单枝通过不同的方法生根。结果表明，在冲洗后10-14天时，叶片上的叶盘在含有10μMα-萘乙酸（NAA）和1μm激动素（10μMα-萘乙酸）的Murashige和Skoog（MS）培养基上从31到37个芽/盘具有芽。 KN）。

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《The Kasetsart Journal》 |1999年第4期|p.486-496|共11页
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Sompong Tachatoe; Osnuma Rurgnoi;
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Direct Shoot Bud Formation and Plantlet Regeneration form Culturing Leaf Discs of Neem (Azadirachta excelsa L.)

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