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首页> 外文期刊>Thin Solid Films >Influence of the surface free energy of silane-coupled mica substrate on the fixing and straightening of DNA
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Influence of the surface free energy of silane-coupled mica substrate on the fixing and straightening of DNA

机译：硅烷偶联云母基底的表面自由能对DNA固定和拉直的影响

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摘要

Methyltrimethoxysilane (MTMS)-coupled mica substrate is reportedly suitable for fixing and straightening of DNA, but 3-aminopropyltriethoxysilane (APTES)-coupled mica substrate has been found less suitable. On MTMS-coupled mica substrate, the straightness of fixed DNA was sufficient, and the adsorption of contaminants was not observed using fluorescence microscopy and atomic force microscopy. For the APTES-coupled mica substrate, however, aggregated or curved DNA and adsorption of contaminants were observed. To clarify the surface factors that are responsible for this suitability, we analyzed the surface free energies of these substrates using the extended Fowkes theory. In each of the surface free energy components, the dispersion force component in the MTMS-coupled mica substrate was lower than that in the APTES-coupled mica substrate. The ratio of the polar force component on the MTMS-coupled mica substrate was about one order of magnitude on the APTES-coupled mica substrate. In addition, the ratio of the hydrogen-bonding force component for the MTMS-coupled mica substrate was about two times larger than that of the APTES-coupled mica substrate. These results suggest that the polar force and hydrogen-bonding force components are important factors for the fixation and straightening of DNA and that the dispersion force components influence the production and adsorption of contaminants.
机译：据报道，甲基三甲氧基硅烷（MTMS）偶联的云母基质适合固定和拉直DNA，但发现3-氨丙基三乙氧基硅烷（APTES）偶联的云母基质较不适合。在MTMS偶联的云母基底上，固定DNA的笔直度足够，并且使用荧光显微镜和原子力显微镜未观察到污染物的吸附。然而，对于APTES偶联的云母底物，观察到聚集的或弯曲的DNA和污染物的吸附。为了弄清造成这种适应性的表面因素，我们使用扩展的Fowkes理论分析了这些基材的表面自由能。在每个表面自由能分量中，MTMS耦合云母基底中的分散力分量低于APTES耦合云母基底中的分散力分量。 MTMS耦合云母基板上的极性力分量之比在APTES耦合云母基板上大约为一个数量级。另外，MTMS偶联的云母基板的氢键合力成分的比例约比APTES偶联的云母基板的氢键合力的比例大两倍。这些结果表明，极性力和氢键力分量是固定和拉直DNA的重要因素，而分散力分量会影响污染物的产生和吸附。

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  • 来源
    《Thin Solid Films》 |2009年第15期|4425-4431|共7页
  • 作者

    Megumi Sasou; Shigeru Sugiyama; Takao Ishida; Toshio Ohtani; Koji Miyake;

  • 作者单位

    Advanced Manufacturing Research Institute (AMRI), National Institute of Advanced Industrial Science and Technology (AIST), Namiki 1-2-1, Tsukuba, Ibaraki, 305-8564, Japan;

    National Food Research Institute (NFRI), National Agriculture and Food Research Organization (NARO), Kannondai 2-1-12, Tsukuba, Ibaraki, 305-8642, Japan;

    Advanced Manufacturing Research Institute (AMRI), National Institute of Advanced Industrial Science and Technology (AIST), Namiki 1-2-1, Tsukuba, Ibaraki, 305-8564, Japan;

    National Food Research Institute (NFRI), National Agriculture and Food Research Organization (NARO), Kannondai 2-1-12, Tsukuba, Ibaraki, 305-8642, Japan;

    Advanced Manufacturing Research Institute (AMRI), National Institute of Advanced Industrial Science and Technology (AIST), Namiki 1-2-1, Tsukuba, Ibaraki, 305-8564, Japan;

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  • 正文语种 eng
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  • 关键词

    DNA; silane; surface free energy; X-ray photoeiectron spectroscopy; fluorescence microscopy; atomic force microcopy;

    机译：脱氧核糖核酸;硅烷表面自由能X射线光电子能谱;荧光显微镜原子力显微镜;

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