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首页> 外文期刊>Thin Solid Films >Patterning Parameters For Biomolecules Microarrays Constructed With Nanosecond And Femtosecond Uv Lasers
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Patterning Parameters For Biomolecules Microarrays Constructed With Nanosecond And Femtosecond Uv Lasers

机译：纳秒和飞秒紫外激光构建的生物分子微阵列的图案化参数

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摘要

The development of laser techniques for transferring biomolecules on solid surfaces in a controlled manner has attracted much attention during the last few years. Laser-induced forward transfer (LIFT) is a direct-write laser technique employed in printing micron-sized patterns or features of a wide range of materials in solid or liquid state, in ambient atmosphere and temperature or in vacuum. In this work, we use LIFT with nanosecond and femtosecond UV lasers for patterning the proteins Biotin, Avidin and Titin. In addition to the laser parameters, we also investigate the influence of the solution viscosity and the receiving surface wettability on the formed patterns. To confirm pattern functionality after transfer, we employ the strategy of patterning a coupling molecule with LIFT, immobilizing an active complementary protein on it and performing a fluorescence microscopy assay afterwards. Our results show that both nanosecond and femtosecond lasers are suitable tools for obtaining micron size protein patterns, with femtosecond laser pulses requiring a lower energy for transfer. We also show that the smallest patterns are formed just above the threshold energy for the thinnest target and that the pattern size increases with increasing surface wettability and decreases with solution viscosity.
机译：在过去的几年中，用于以受控方式将生物分子转移到固体表面上的激光技术的发展引起了很多关注。激光感应正向转移（LIFT）是一种直接写入激光技术，用于在环境大气和温度下或在真空中以固态或液态打印微米级图案或各种材料的特征。在这项工作中，我们将LIFT与纳秒和飞秒UV激光器一起使用，以对蛋白质Biotin，Avidin和Titin进行图案化。除激光参数外，我们还研究了溶液粘度和接收表面润湿性对所形成图案的影响。为了确认转移后的模式功能，我们采用了用LIFT模式化偶联分子，将活性互补蛋白固定在其上并随后进行荧光显微镜检测的策略。我们的结果表明，纳秒和飞秒激光都是获得微米尺寸蛋白质图案的合适工具，飞秒激光脉冲需要较低的能量进行转移。我们还表明，最小的图形刚好在最薄目标的阈值能量之上，并且图形尺寸随表面润湿性的增加而增加，而随溶液粘度的减小而减小。

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