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首页> 外文期刊>Fluorescein Diacetate for Determination of Cell Viability in Tissue-Engineered Skin >Measuring Angiogenic Cytokines, Circulating Endothelial Cells, and Endothelial Progenitor Cells in Peripheral Blood and Cord Blood: VEGF and CXCL12 Correlate with the Number of Circulating Endothelial Progenitor Cells in Peripheral Blood
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Measuring Angiogenic Cytokines, Circulating Endothelial Cells, and Endothelial Progenitor Cells in Peripheral Blood and Cord Blood: VEGF and CXCL12 Correlate with the Number of Circulating Endothelial Progenitor Cells in Peripheral Blood

机译：测量外周血和脐带血中的血管生成细胞因子，循环内皮细胞和内皮祖细胞：VEGF和CXCL12与外周血循环内皮祖细胞的数量相关

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Circulating endothelial cells (CECs) and endothelial progenitor cells (EPCs) are thought to play an important role in the vascularization of damaged tissues and cancers. These cells are also required for tissue-engineered blood vessels and to help skin substitutes revascularize more efficiently. A standard approach to the phenotyping and enumeration of CEC and EPC is key to the development of new therapies, and the identification of biomarkers within the blood that regulate their levels may be important for the treatment of cancer. We have devised an improved multiparameter flow cytometric assay for CEC and circulating EPC enumeration. This assay uses antibodies recognizing CD133 and CD34 to identify EPC and CEC, respectively, and incorporates specific markers CD144 and vascular endothelial growth factor receptor 2 (VEGFR-2) for both CEC and EPC cells. In peripheral blood (PB), mean CEC numbers were 55 ± 95 mL−1 and mean EPC numbers were 44 ± 58 mL−1 (n = 60). We also found a significant correlation of both plasma VEGF (r = 0.90, p < 0.001) and CXCL12 (r = 0.84, p < 0.001) with EPCs, but not CECs. The cytokines also correlated with each other (r = 0.85, p < 0.001). In umbilical cord blood (UCB) we found on average 13 times more CEC (719 ± 338 mL−1) and 7 times more EPC (299 ± 245 mL−1) than in PB. However, serum VEGF and CXCL12 levels in UCB did not correlate with either EPC or CEC numbers. These results suggest a major role for VEGF and CXCL12 in the control of marrow-derived EPCs in adult PB and provide normal data for comparison with patient populations
机译：循环内皮细胞（CEC）和内皮祖细胞（EPC）被认为在受损组织和癌症的血管形成中起着重要作用。这些细胞也是组织工程血管所需的，有助于皮肤替代品更有效地血管重建。 CEC和EPC的表型和枚举的标准方法是开发新疗法的关键，血液中调节其水平的生物标志物的鉴定对于癌症的治疗可能很重要。我们为CEC和循环EPC枚举设计了一种改进的多参数流式细胞术。该测定法使用识别CD133和CD34的抗体分别识别EPC和CEC，并为CEC和EPC细胞掺入了特异性标记CD144和血管内皮生长因子受体2（VEGFR-2）。在外周血（PB）中，平均CEC数为55±95 mL-1，平均EPC数为44±58 mL-1（n = 60）。我们还发现血浆VEGF（r = 0.90，p <0.001）和CXCL12（r = 0.84，p <0.001）与EPCs显着相关，而与CECs没有显着相关性。细胞因子也相互关联（r = 0.85，p <0.001）。在脐带血（UCB）中，我们发现CEC（719±338 mL-1）平均比PB高13倍，EPC（299±245 mL-1）高7倍。但是，UCB中的血清VEGF和CXCL12水平与EPC或CEC数均无关。这些结果表明，VEGF和CXCL12在成人PB的骨髓源性EPC的控制中起主要作用，并提供正常数据与患者人群进行比较

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