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首页> 外文期刊>Tissue Engineering Part A >Functional Tissue-Engineered Valves from Cell-Remodeled Fibrin with Commissural Alignment of Cell-Produced Collagen
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Functional Tissue-Engineered Valves from Cell-Remodeled Fibrin with Commissural Alignment of Cell-Produced Collagen

机译：细胞改建的纤维蛋白的功能性组织工程瓣膜，具有细胞产生的胶原的连合排列方式

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摘要

Heart valve replacements composed of living tissue that can adapt, repair, and grow with a patient would provide a more clinically beneficial option than current inert replacements. Bioartificial valves were produced by entrapping human dermal fibroblasts within a fibrin gel. Using a mold design that presents appropriate mechanical constraints to the cell-induced fibrin gel compaction, gross fiber alignment (commissure-to-commissure alignment in the leaflets and circumferential alignment in the root) and the basic geometry of a native aortic valve were obtained. After static incubation on the mold in complete medium supplemented with transforming growth factor beta 1, insulin, and ascorbate, collagen fibers produced by the entrapped cells were found to coalign with the fibrin based on histological analyses. The resultant tensile mechanical properties were anisotropic. Ultimate tensile strength and tensile modulus of the leaflets in the commissural direction were 0.53 and 2.34 MPa, respectively. The constructs were capable of withstanding backpressure commensurate with porcine aortic valves in regurgitation tests (330 mmHg) and opened and closed under physiological pressure swings of 10 and 20 mmHg, respectively. These data support proof of principle of using cell-remodeled fibrin gel to produce tissue-engineered valve replacements.
机译：由能与患者适应，修复和成长的活体组织组成的心脏瓣膜置换术将比目前的惰性置换术提供更有益于临床的选择。通过将人皮肤成纤维细胞包埋在纤维蛋白凝胶中来生产生物人工瓣膜。使用对细胞诱导的纤维蛋白凝胶压实表现出适当机械约束的模具设计，可获得总纤维排列（小叶中的连合排列和根部的周向排列）和天然主动脉瓣的基本几何形状。在补充有转化生长因子β1，胰岛素和抗坏血酸的完全培养基中在模具上静态孵育后，根据组织学分析，发现被截留的细胞产生的胶原纤维与纤维蛋白共对齐。所得的拉伸机械性能是各向异性的。小叶在接合方向上的极限抗拉强度和拉伸模量分别为0.53和2.34 MPa。该构造物能够在反流试验（330 mmHg）中承受与猪主动脉瓣相当的背压，并分别在10和20 mmHg的生理压力波动下打开和关闭。这些数据支持使用细胞改建的纤维蛋白凝胶生产组织工程瓣膜替代物的原理证明。

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  • 来源
    《Tissue Engineering Part A》 |2008年第1期|83-95|共13页
  • 作者

    Paul S. Robinson; Sandra L. Johnson; Michael C. Evans; Victor H. Barocas; Robert T. Tranquillo;

  • 作者单位

    Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota.;

    Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota.;

    Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota.;

    Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota.;

    Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota.|Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota.;

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