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首页> 外文期刊>Translational Stroke Research >Granulocyte-colony Stimulating Factor in Combination with Stem Cell Factor Confers Greater Neuroprotection after Hypoxic–Ischemic Brain Damage in the Neonatal Rats than a Solitary Treatment
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Granulocyte-colony Stimulating Factor in Combination with Stem Cell Factor Confers Greater Neuroprotection after Hypoxic–Ischemic Brain Damage in the Neonatal Rats than a Solitary Treatment

机译：粒细胞集落刺激因子与干细胞因子联合给予新生大鼠缺氧缺血性脑损伤后比单独治疗具有更大的神经保护作用

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Neonatal hypoxia–ischemia (HI) is a devastating condition resulting in neuronal cell death and often culminates in neurological deficits. Granulocyte-colony stimulating factor (G-CSF) has been shown to have neuroprotective activity via inhibition of apoptosis and inflammation in various stroke models. Stem cell factor (SCF) regulates hematopoietic stem cells in the bone marrow and has been reported to have neuroprotective properties in an experimental ischemic stroke model. In this study, we aim to determine the protective effects of G-CSF in combination with SCF treatment after experimental HI. Seven-day-old Sprague–Dawley rats were subjected to unilateral carotid artery ligation followed by 2.5 h of hypoxia. Animals were randomly assigned to five groups: Sham (n = 8), Vehicle (n = 8), HI with G-CSF treatment (n = 9), HI with SCF treatment (n = 9), and HI with G-CSF + SCF treatment (coadministration group; n = 10). G-CSF (50 μg/kg), SCF (50 μg/kg), and G-CSF + SCF (50 μg/kg) were administered intraperitoneally 1 h post HI followed by daily injection for 4 consecutive days (five total injections). Animals were euthanized 14 days after HI for neurological testing. Additionally, assessment of brain, heart, liver, spleen, and kidney atrophy was performed. Both G-CSF and G-CSF + SCF treatments improved body growth and decreased brain atrophy at 14 days post HI. No significant differences were found in the peripheral organ weights between groups. Finally, the G-CSF + SCF coadministration group showed significant improvement in neurological function. Our data suggest that administration of G-CSF in combination with SCF not only prevented brain atrophy but also significantly improved neurological function.
机译：新生儿缺氧缺血（HI）是一种破坏性疾病，会导致神经元细胞死亡，并经常导致神经功能缺陷。粒细胞集落刺激因子（G-CSF）已显示出通过抑制各种中风模型中的细胞凋亡和炎症而具有神经保护活性。干细胞因子（SCF）调节骨髓中的造血干细胞，并已报道在实验性缺血性中风模型中具有神经保护特性。在这项研究中，我们旨在确定实验性HI后联合SCF处理G-CSF的保护作用。对7天大的Sprague–Dawley大鼠进行单侧颈动脉结扎，然后缺氧2.5小时。将动物随机分为五组：假手术（n = 8），媒介物（n = 8），G-CSF处理的HI（n = 9），SCF处理的HI（n = 9）和G-CSF HI + SCF治疗（联合用药组； n = 10）。 HI后1 h腹腔内给予G-CSF（50μg/ kg），SCF（50μg/ kg）和G-CSF + SCF（50μg/ kg），然后连续4天每天注射（共5次注射） 。 HI后14天对动物实施安乐死以进行神经学测试。此外，还进行了大脑，心脏，肝脏，脾脏和肾脏萎缩的评估。 HI后14天，G-CSF和G-CSF + SCF治疗均可改善机体生长并减少脑萎缩。各组之间末梢器官重量无明显差异。最后，G-CSF + SCF共同给药组显示出神经功能的显着改善。我们的数据表明，与SCF联合使用G-CSF不仅可以预防脑萎缩，还可以显着改善神经功能。

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