...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Translational Stroke Research >Assessing the Quality and Reproducibility of a Proteomic Platform for Clinical Stroke Biomarker Discovery
【24h】

Assessing the Quality and Reproducibility of a Proteomic Platform for Clinical Stroke Biomarker Discovery

机译：评估蛋白质组学平台用于临床卒中生物标记物发现的质量和可重复性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to investigate the quality and reproducibility of mass spectra derived from a matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) platform in a patient population undergoing carotid endarterectomy. Plasma samples were either digested with trypsin or left undigested, fractionated with either C18 or weak cation exchange (WCX) columns and analysed by MALDI-TOF MS. Quality of mass spectra for each method was assessed by baseline correction (lower area under the curve ratio indicating higher quality) and signal-to-noise ratio. Mean coefficient of variation (CV%) assessed reproducibility between repeated experiments and methods. Identified mass peak intensity differences were assessed for consistency across repeated experiments. Plasma from six patients was analysed. The quality of mass spectra was significantly better when derived from digested plasma fractionated by either WCX or C18 methods compared to undigested plasma fractionated by WCX (analysis of variance, p < 0.05). Mean CV% for repeated experiments was 18% and 28% for WCX and C18 fractionated digested plasma, respectively. A small number of differences in mass peak intensities were consistently observed in repeated experiments. Repeated experiments are required to confidently identify non-random mass peak intensity differences as putative plasma biomarkers that merit further investigation.
机译：这项研究的目的是研究在接受颈动脉内膜切除术的患者人群中，从基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）平台获得的质谱的质量和重现性。血浆样品用胰蛋白酶消化或不消化，用C18色谱柱或弱阳离子交换（WCX）柱进行分馏，并通过MALDI-TOF MS分析。通过基线校正（曲线比率下的较小区域表示较高的质量）和信噪比来评估每种方法的质谱质量。平均变异系数（CV％）评估了重复实验和方法之间的可重复性。在重复的实验中评估鉴定出的质量峰强度差异的一致性。分析了六名患者的血浆。当通过WCX或C18方法分馏的消化血浆得到的质谱图相比，通过WCX分馏的未消化血浆得到的质谱质量更好（方差分析，p <0.05）。重复实验的平均CV％对于WCX和C18分级消化的血浆分别为18％和28％。在重复实验中始终观察到质量峰强度的少量差异。需要重复实验才能确信地确定非随机质谱峰强度差异，作为值得推崇的血浆生物标志物。

著录项

  • 来源
    《Translational Stroke Research》 |2010年第4期|304-314|共11页
  • 作者

    Ediri Sideso; Michalis Papadakis; Cynthia Wright; Ashok Handa; Alastair Buchan; Benedikt Kessler; James Kennedy;

  • 作者单位

    Acute Stroke Programme Nuffield Department of Medicine University of Oxford Oxford UK;

    Acute Stroke Programme Nuffield Department of Medicine University of Oxford Oxford UK;

    Central Proteomics Facility Henry Wellcome Building for Molecular Physiology Nuffield Department of Medicine University of Oxford Oxford UK;

    Nuffield Department of Surgery John Radcliffe Hospital University of Oxford Oxford UK;

    Acute Stroke Programme Nuffield Department of Medicine University of Oxford Oxford UK;

    Central Proteomics Facility Henry Wellcome Building for Molecular Physiology Nuffield Department of Medicine University of Oxford Oxford UK;

    Acute Stroke Programme Nuffield Department of Medicine University of Oxford Oxford UK;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cerebral Ischaemia; Proteomics; Biomarker discovery; MALDI-TOF; C18; Weak cation exchange;

    机译：脑缺血;蛋白质组学;生物标志物的发现;MALDI-TOF;C18;弱阳离子交换;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号