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PCR test for detecting Taenia solium cysticercosis in pig carcasses

机译：PCR检测猪car体Ta虫囊虫病

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摘要

Polymerase chain reaction (PCR) test was employed to detect Taenia solium DNA in muscle lesions for validation of the meat inspection results of slaughtered pigs. Two sets of oligonucleotide primers, one targeted against the large subunit rRNA gene (TBR primers) and the other targeted against cytochrome c oxidase subunit 1 gene (Cox1 primers) of T. solium were used in this study. On reactivity in PCR test, the TBR primers and the Cox1 primers yielded products of 286 and 984 bp, respectively, in cysticercosis positive cases. Both the sets of primers were found to be highly specific, since they did not yield any PCR product in negative controls. A total of 225 pig carcasses were screened for cysticercosis by meat inspection, out of which 25 carcasses with visible cysts (16 viable and 9 degenerated cysts) were also confirmed to be positive for cysticercosis in PCR test. However, out of the 35 carcasses with suspected lesions on meat inspection, only two were found to be positive for cysticercosis in PCR test. The detection limits for both the primer sets were analyzed. The TBR primer set could detect up to 10 pg of cysticercus DNA, whereas the Cox1 primer set could detect only up to 1 ng. It is evident from the study that PCR test is an efficient tool for validation of meat inspection results and also to rule out ambiguity in carcass judgment of suspected cases of porcine cysticercosis.
机译：采用聚合酶链反应（PCR）检测肌肉损伤中的Ta虫so虫DNA，以验证屠宰猪的肉质检查结果。本研究使用了两组寡核苷酸引物，一组针对大亚基rRNA基因（TBR引物），另一组针对T. solium的细胞色素C氧化酶亚基1基因（Cox1引物）。在PCR试验中的反应性上，在囊尾rc病阳性病例中，TBR引物和Cox1引物分别产生286和984 bp的产物。发现两组引物都是高度特异性的，因为它们在阴性对照中均不产生任何PCR产物。通过肉类检查共筛选了225头猪pig体的囊尾rc病，其中25具可见囊肿的（体（16个有生存力的囊肿和9个退化的囊肿）在PCR试验中也被证实为囊尾rc病阳性。然而，在35具肉类检查中被怀疑有损伤的尸体中，在PCR试验中仅发现2例为囊虫病阳性。分析了两种引物组的检测限。 TBR引物组最多可检测10 pg的囊性囊菌DNA，而Cox1引物组最多可检测1 ng。从这项研究中可以明显看出，PCR检测是验证肉类检验结果的有效工具，也可以排除对猪囊尾rc病疑似病例的car体判断的歧义。

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  • 来源
    《Tropical Animal Health and Production》 |2012年第1期|95-99|共5页
  • 作者

    Chennuru Sreedevi; Mohammad Hafeez; Putcha Anand Kumar; Vukka Chengalva Rayulu; Kothapalli Venkata Subramanyam; Krovvidi Sudhakar;

  • 作者单位

    Department of Veterinary Parasitology College of Veterinary Science Sri Venkateswara Veterinary University Tirupati 517 502 Andhra Pradesh India;

    Department of Veterinary Parasitology College of Veterinary Science Sri Venkateswara Veterinary University Tirupati 517 502 Andhra Pradesh India;

    Department of Veterinary Microbiology NTR College of Veterinary Science Sri Venkateswara Veterinary University Gannavaram 521102 Andhra Pradesh India;

    Department of Veterinary Parasitology College of Veterinary Science Sri Venkateswara Veterinary University Tirupati 517 502 Andhra Pradesh India;

    Department of Veterinary Microbiology NTR College of Veterinary Science Sri Venkateswara Veterinary University Gannavaram 521102 Andhra Pradesh India;

    Department of Animal Genetics ampamp Breeding NTR College of Veterinary Science Sri Venkateswara Veterinary University Gannavaram 521102 Andhra Pradesh India;

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  • 正文语种 eng
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  • 关键词

    Cysticercus cellulosae; Detection limits; Oligonucleotide primers; PCR; Porcine cysticercosis; Taenia solium;

    机译：猪囊尾囊;检测限;寡核苷酸引物;PCR;猪囊尾osis病;Ta虫;

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