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Molecular typing of Brucella species isolates from livestock and human

机译:来自家畜和人类的布鲁氏菌种的分子分型

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Although host specificity has been observed in different species of Brucella, crossing the animal host boundary is likely to occur at any time. In this study, Bruce ladder PCR and abortus–melitensis–ovis–suis (AMOS) PCR assays were used to characterize 47 Brucella isolates from Indian origin in order to know exact species for understanding epidemiology of brucellosis. Out of them, 28, 14, and 5 isolates were found to be Brucella abortus, Brucella melitensis, and Brucella suis, respectively. Further analysis by AMOS PCR has identified that all the B. abortus isolates belong to any one of the biovar 1, 2, or 4; of the five B. suis isolates, three belong to biovar 1 and two belong to any one of the biovar 2, 3, 4, or 5. Although this multiplex Bruce ladder PCR is useful in differentiating all Brucella species, elaborate study is required to further characterize the isolates at exact biovar level.
机译:尽管已在布鲁氏菌的不同物种中观察到宿主特异性,但可能随时会越过动物宿主边界。在这项研究中,使用布鲁斯梯形PCR和流产-melitensis-ovis-suis(AMOS)PCR分析法鉴定了47种来自印度的布鲁氏菌分离株,以了解了解布鲁氏菌病流行病学的确切物种。其中有28、14和5个分离株分别是流产布鲁氏菌,布鲁氏布鲁氏菌和猪布鲁氏菌。通过AMOS PCR的进一步分析已确定,所有流产双歧杆菌均属于biovar 1、2或4之一。在这五个猪双歧杆菌分离物中,三个属于生物变种1,两个属于生物变种2、3、4或5中的任何一个。尽管这种多重布鲁斯阶梯PCR可用于区分所有布鲁氏菌种,但仍需进行详尽的研究进一步以确切的生物var水平表征分离株。

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