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Maedi in slaughtered sheep: A pathology and polymerase chain reaction study in southwestern Iran

机译：宰羊中的前田：伊朗西南部的病理和聚合酶链反应研究

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摘要

Maedi-visna (MV) is an important slow viral disease of sheep leading to a progressive lymphoproliferative disease. It affects multiple organs primarily the lungs, where it causes interstitial pneumonia (maedi). In this study, the lungs of 1,000 sheep carcasses were grossly inspected and those suspected to have maedi were studied at histopathological and molecular levels. A polymerase chain reaction (PCR) technique that amplified a 291-base pair DNA in the long terminal repeat (LTR) sequence of MV provirus was conducted on all the 50 suspected lungs together with 10 normal appearing lungs as controls. Amplicons of the expected size were detected in 11 (n = 11/50) suspected sheep, and one of the 10 control sheep. Histopathologic study of the pulmonary lesions of all 11 (n = 11/11) positive sheep showed MV lesions, including hyperplasia of the perivascular and peribronchiolar lymphoid cells, interstitial lymphoplasmacytic infiltration and smooth muscle hyperplasia and the histopathologic findings were correlated with PCR results. In contrast, the tissue sections of control animals were almost normal at histopathological level; however, PCR technique demonstrated that one of them was affected by maedi. This study showed that the LTR-PCR had high specificity and sensitivity in diagnosis of this viral infection. This study is the first to evaluate the prevalence of MV virus infection in sheep in Iran.
机译：马迪-维斯纳病毒（MV）是一种重要的绵羊慢病毒病，会导致进行性淋巴细胞增生性疾病。它会影响主要是肺的多个器官，在肺中会引起间质性肺炎（maedi）。在这项研究中，对1000只绵羊尸体的肺进行了全面检查，并对那些怀疑患有前足动物的肺进行了组织病理学和分子水平的研究。聚合酶链反应（PCR）技术在MV前病毒的长末端重复（LTR）序列中扩增了291个碱基对的DNA，并在所有50个可疑肺部和10个正常出现的肺部进行了对照。在11只（n = 11/50）可疑绵羊和10只对照绵羊之一中检出了预期大小的扩增子。对所有11只（n = 11/11）阳性绵羊的肺部病变的组织病理学研究均显示MV病变，包括血管周和支气管周淋巴样细胞增生，间质性淋巴胞浆浸润和平滑肌增生，并且组织病理学发现与PCR结果相关。相反，对照动物的组织切片在组织病理学水平上几乎是正常的。然而，PCR技术证明其中之一受到前调的影响。这项研究表明，LTR-PCR对这种病毒感染的诊断具有很高的特异性和敏感性。这项研究是第一个评估伊朗绵羊MV病毒感染率的研究。

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  • 来源
    《Tropical Animal Health and Production》 |2012年第1期|113-118|共6页
  • 作者

    Shahrzad Azizi; Elahe Tajbakhsh; Farzad Fathi; Ahmad Oryan; Hassan Momtaz; Mehdi Goodarzi;

  • 作者单位

    Department of Pathology School of Veterinary Medicine Islamic Azad University Shahrekord Branch Shahrekord Iran;

    Department of Microbiology School of Basic Sciences Islamic Azad University Shahrekord Branch Shahrekord Iran;

    Graduated of Veterinary Medicine Islamic Azad University Shahrekord Branch Shahrekord Iran;

    Department of Veterinary Pathology School of Veterinary Medicine Shiraz University Shiraz Iran;

    Department of Microbiology School of Veterinary Medicine Islamic Azad University Shahrekord Branch Shahrekord Iran;

    Department of Clinical Sciences School of Veterinary Medicine Islamic Azad University Shahrekord Branch Shahrekord Iran;

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  • 正文语种 eng
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  • 关键词

    Maedi–visna; Histopathology; PCR; Ovine progressive pneumonia;

    机译：Maedi-visna;组织病理学;PCR;绵羊进行性肺炎;

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