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首页> 外文期刊>Tropical Animal Health and Production >In vitro evaluation, in vivo quantification, and microbial diversity studies of nutritional strategies for reducing enteric methane production
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In vitro evaluation, in vivo quantification, and microbial diversity studies of nutritional strategies for reducing enteric methane production

机译：减少肠内甲烷产生的营养策略的体外评估，体内定量和微生物多样性研究

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摘要

The main objective of the present work was to study nutritive strategies for lessening the CH4 formation associated to ruminant tropical diets. In vitro gas production technique was used for evaluating the effect of tannin-rich plants, essential oils, and biodiesel co-products on CH4 formation in three individual studies and a small chamber system to measure CH4 released by sheep for in vivo studies was developed. Microbial rumen population diversity from in vitro assays was studied using qPCR. In vitro studies with tanniniferous plants, herbal plant essential oils derived from thyme, fennel, ginger, black seed, and Eucalyptus oil (EuO) added to the basal diet and cakes of oleaginous plants (cotton, palm, castor plant, turnip, and lupine), which were included in the basal diet to replace soybean meal, presented significant differences regarding fermentation gas production and CH4 formation. In vivo assays were performed according to the results of the in vitro assays. Mimosa caesalpineaefolia, when supplemented to a basal diet (Tifton-85 hay Cynodon sp, corn grain, soybean meal, cotton seed meal, and mineral mixture) fed to adult Santa Ines sheep reduced enteric CH4 emission but the supplementation of the basal diet with EuO did not affect (P > 0.05) methane released. Regarding the microbial studies of rumen population diversity using qPCR with DNA samples collected from the in vitro trials, the results showed shifts in microbial communities of the tannin-rich plants in relation to control plant. This research demonstrated that tannin-rich M. caesepineapholia, essential oil from eucalyptus, and biodiesel co-products either in vitro or in vivo assays showed potential to mitigate CH4 emission in ruminants. The microbial community study suggested that the reduction in CH4 production may be attributed to a decrease in fermentable substrate rather than to a direct effect on methanogenesis.
机译：本研究的主要目的是研究减少反刍热带饮食中CH4 形成的营养策略。在三项独立研究中，采用体外产气技术评估了单宁含量高的植物，精油和生物柴油副产物对CH4 形成的影响，并采用小室系统测量了由CH4 释放的CH4 。开发了用于体内研究的绵羊。使用qPCR研究了来自体外测定的微生物瘤胃种群多样性。对单宁植物，百里香，茴香，生姜，黑种子和桉树油（EuO）衍生的草本植物精油进行体外研究，并将其添加到含油植物（棉花，棕榈，蓖麻，萝卜和羽扇豆）的基础饮食和蛋糕中）（包括在基础饮食中以代替豆粕）在发酵气体产生和CH4 形成方面存在显着差异。根据体外测定的结果进行体内测定。如果将含羞草含羞草（Mimosa caesalpineaefolia）添加到成年圣塔伊内斯绵羊的基础日粮中（Tifton-85干草Cynodon sp，玉米籽粒，大豆粉，棉籽粉和矿物质混合物），可减少肠内CH4的排放，但是补充含EuO的基础饮食不影响（P> 0.05）甲烷的释放。关于使用从体外试验收集的DNA样品进行qPCR进行瘤胃群体多样性的微生物研究，结果表明，单宁含量高的植物的微生物群落相对于对照植物发生了变化。这项研究表明，富含单宁的M. caesepineapholia，桉树的精油和生物柴油副产物，无论在体内还是体外试验中均显示出减轻反刍动物CH4排放的潜力。微生物群落研究表明，CH4 产生的减少可能归因于可发酵底物的减少，而不是直接影响产甲烷作用。

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