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首页> 外文期刊>Tropical Animal Health and Production >Prevalence, molecular fingerprinting and drug resistance profile of enterovirulent Escherichia coli isolates from free-ranging yaks of Tawang district, Arunachal Pradesh, India
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Prevalence, molecular fingerprinting and drug resistance profile of enterovirulent Escherichia coli isolates from free-ranging yaks of Tawang district, Arunachal Pradesh, India

机译：印度阿鲁纳恰尔邦塔旺地区散养牛肠病毒致病性大肠杆菌分离株的流行，分子指纹图谱和耐药性谱

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摘要

Of 273 samples (rectal swab) collected from free-ranging yaks of Tawang district, Arunachal Pradesh, 42 Shiga toxin-producing Escherichia coli (STEC), six enteropathogenic E. coli (EPEC) and 27 enterotoxigenic E. coli (ETEC) strains were isolated. All the STEC and EPEC strains were further investigated for respective stx variants (for STEC only) and additional putative virulence factors. The 27 ETEC strains were also screened for characteristic enterotoxin gene(s) and colonization factors. Occurrence of ETEC was significantly (p < 0.05) higher in the diarrheic yaks and yaks of less than 1 year of age. Majority of enterovirulent E. coli isolates were resistant to amikacin, azithromycin, chloramphenicol, colistin, doxycycline, furazolidone, nalidixic acid, nitrofurantoin, streptomycin and tetracycline. Dendrogram, constructed with molecular fingerprinting profiles obtained from RAPD (Randomly Amplified Polymorphic DNA) and ERIC (Enterobacterial Repetitive Intergenic Consensus) PCR, placed the isolates in different clusters irrespective of their serotypes, virulence gene and drug resistance pattern. Collectively, the study indicates that yaks, being a potential reservoir of multidrug resistant STEC and EPEC, may represent significant risk to public health in this region. Higher recovery of ETEC isolates from yaks with diarrhea points out that ETEC may be a major determinant for repeated occurrence of diarrhea in yaks.
机译：从阿鲁纳恰尔邦塔旺地区放养的牛采集的273份样品（直肠拭子）中，有42株产生志贺毒素的大肠杆菌（STEC），6株致肠毒素的大肠杆菌（EPEC）和27株产肠毒素的大肠杆菌（ETEC）隔离的。进一步研究了所有STEC和EPEC菌株的stx变异（仅适用于STEC）和其他假定的毒力因子。还对27株ETEC菌株进行了特征性肠毒素基因和定居因子的筛选。腹泻牛和小于1岁的牛中ETEC的发生率显着更高（p <0.05）。多数肠毒性大肠杆菌分离株对丁胺卡那霉素，阿奇霉素，氯霉素，粘菌素，强力霉素，呋​​喃唑酮，萘啶酸，呋喃妥因，链霉素和四环素具有抗性。用从RAPD（随机扩增多态性DNA）和ERIC（肠细菌重复性基因间共识）PCR获得的分子指纹图谱构建的树状图，将分离物置于不同的簇中，无论其血清型，毒力基因和耐药模式如何。总体而言，研究表明indicates牛是耐多药STEC和EPEC的潜在库，可能对该地区的公共卫生构成重大风险。从腹泻的ks牛中分离出的ETEC菌株的回收率更高，这表明ETEC可能是repeated牛中反复出现腹泻的主要决定因素。

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  • 来源
    《Tropical Animal Health and Production》 |2012年第5期|1063-1072|共10页
  • 作者

    Samiran Bandyopadhyay; Chandan Lodh; Mihir Sarkar; Monoj K. Ghosh; Asit K. Bera; Debasish Bhattacharyya; Dipak K. Mondal; Kishore K. Baruah;

  • 作者单位

    National Research Centre on Yak ICAR Dirang 790101 West Kameng Arunachal Pradesh India;

    West Bengal University of Animal and Fishery Sciences 37 ampamp 68 K B Sarani Kolkata 700037 West Bengal India;

    National Research Centre on Yak ICAR Dirang 790101 West Kameng Arunachal Pradesh India;

    National Research Centre on Yak ICAR Dirang 790101 West Kameng Arunachal Pradesh India;

    National Research Centre on Yak ICAR Dirang 790101 West Kameng Arunachal Pradesh India;

    National Research Centre on Yak ICAR Dirang 790101 West Kameng Arunachal Pradesh India;

    Indian Veterinary Research Institute ERS Kolkata 700037 West Bengal India;

    National Research Centre on Yak ICAR Dirang 790101 West Kameng Arunachal Pradesh India;

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  • 正文语种 eng
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  • 关键词

    STEC; EPEC; ETEC; RAPD; ERIC; Drug resistance; Yak; India;

    机译：STEC;EPEC;ETEC;RAPD;ERIC;耐药性;Y牛;印度;

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