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首页> 外文期刊>Tropical Animal Health and Production >In vitro antimicrobial activity of Combretum molle (Combretaceae) against Staphylococcus aureus and Streptococcus agalactiae isolated from crossbred dairy cows with clinical mastitis
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In vitro antimicrobial activity of Combretum molle (Combretaceae) against Staphylococcus aureus and Streptococcus agalactiae isolated from crossbred dairy cows with clinical mastitis

机译：Combrtum molle（Combretaceae）对分离自患有临床乳腺炎的杂交奶牛的金黄色葡萄球菌和无乳链球菌的体外抗菌活性

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摘要

Following the rapidly expanding dairy enterprise, mastitis has remained the most economically damaging disease. The objective of this study was mainly to investigate the in vitro antibacterial activities of ethanol extracts of Combretum molle (R.Br.Ex.G.Don) Engl & Diels (Combretaceae) against antibiotic-resistant and susceptible Staphylococcus aureus and Streptococcus agalactiae isolated from clinical cases of bovine mastitis using agar disc diffusion method. The leaf and bark extracts showed antibacterial activity against S. aureus at concentrations of 3 mg/ml while the stem and seed extract did not show any bioactivity. Although both leaf and bark extracts were handled in the same manner, the antibacterial activity of the bark extract against the bacterial strains had declined gradually to a lower level as time advanced after extraction. The leaf extract had sustained bioactivity for longer duration. The susceptibility of the bacteria to the leaf extract is not obviously different between S. aureus and S. agalactiae. Also, there was no difference in susceptibility to the leaf extract between the antibiotic-resistant and antibiotic-sensitive bacteria. Further phytochemical and in vivo efficacy and safety studies are required to evaluate the therapeutic value of the plant against bovine mastitis.
机译：随着乳品企业的迅速发展，乳腺炎仍然是最具经济破坏力的疾病。这项研究的主要目的是研究Combretum molle（R.Br.Ex.G.Don）Engl＆Diels（Combretaceae）乙醇提取物对从中分离的耐抗生素和敏感的金黄色葡萄球菌和无乳链球菌的体外抗菌活性。牛乳腺炎的临床病例采用琼脂圆盘扩散法。叶和树皮提取物在浓度为3 mg / ml时对金黄色葡萄球菌具有抗菌活性，而茎和种子提取物未显示任何生物活性。尽管叶和树皮提取物都以相同的方式处理，但随着提取时间的延长，树皮提取物对细菌菌株的抗菌活性逐渐下降至较低水平。叶提取物具有持续较长时间的生物活性。金黄色葡萄球菌和无乳链球菌之间细菌对叶提取物的敏感性没有明显差异。而且，在抗药性细菌和对抗生素敏感的细菌之间对叶提取物的敏感性没有差异。需要进一步的植物化学和体内功效及安全性研究来评估植物对牛乳腺炎的治疗价值。

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