• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Tropical Animal Health and Production >Comparison of conventional and non-conventional techniques for the diagnosis of bovine brucellosis in Sudan
【24h】

Comparison of conventional and non-conventional techniques for the diagnosis of bovine brucellosis in Sudan

机译：苏丹牛布鲁氏菌病常规与非常规技术的比较

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The objective of the present study was to detect brucellosis in suspected dairy cattle in Khartoum State, Sudan using the conventional serological tests and tests done on milk in comparison to a PCR-based technique. Milk and blood samples collected simultaneously from suspected brucellosis cows (n = 147) in 12 different dairy farms around Khartoum State were used in the study. Overall, 54 (36.7%) of the total milk samples were positive according to the milk ring test (MRT), while 29 (19.7%) of the serum samples were positive according to the Rose Bengal test (RBT); microscopy on modified Ziehl–Neelsen-stained slides detected 13.6% of the cases, and recovery of Brucella species on both Brucella medium and tryptic soya agar was 7.5%. Thirty-three (22.4%) samples were found positive on PCR-amplified IS711 which were then taken as positive brucellosis cases. The differences of RBT and PCR-IS711 from MRT were highly significant (P < 0.05). MRT detected more cases of bovine brucellosis compared to RBT, PCR, microscopy, and culture. MRT is recommended as a noninvasive test compared to RBT, and it is less expensive compared to PCR and culture.
机译：本研究的目的是使用常规血清学检测和与基于PCR的技术相比对牛奶进行的检测来检测苏丹喀土穆州可疑奶牛的布鲁氏菌病。该研究使用了从喀土穆州周围12个不同奶牛场同时从疑似布鲁氏菌病奶牛（n = 147）中采集的牛奶和血液样本。总体而言，根据乳环试验（MRT），所有牛奶样品中有54（36.7％）呈阳性，而根据Rose Bengal试验（RBT），有29（19.7％）血清样品呈阳性。修改后的Ziehl–Neelsen染色载玻片的显微镜检测结果显示，有13.6％的病例，布鲁氏菌培养基和胰蛋白酶大豆琼脂上的布鲁氏菌物种回收率为7.5％。发现33份（22.4％）样本在PCR扩增的IS711上呈阳性，然后被视为布鲁氏菌病阳性病例。 MRT的RBT和PCR-IS711差异非常显着（P <0.05）。与RBT，PCR，显微镜和培养相比，MRT检测到更多的牛布鲁氏菌病病例。与RBT相比，推荐将MRT作为一种非侵入性测试，并且与PCR和培养相比，它更便宜。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号