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首页> 外文期刊>Tropical Animal Health and Production >Comparative sequence analysis of B5R gene of zoonotic buffalo pox virus isolates with other orthopoxviruses
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Comparative sequence analysis of B5R gene of zoonotic buffalo pox virus isolates with other orthopoxviruses

机译：人畜共患水牛痘病毒分离株与其他正痘病毒B5R基因的比较序列分析

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摘要

The present paper describes the isolation of buffalo pox virus from scab lesions and its molecular characterization through B5R gene sequencing. During our study, pustular pox lesions were observed on the teats and mammary parenchyma of cattle and buffaloes, and the disease was of significant zoonotic importance since similar lesions were produced on the hands, legs, and face of people in close contact with the affected animals. The collected scab materials were subjected for virus isolation in 9–11-day-old chicken embryos by the chorioallontoic membrane route and in the Vero cell line. The virus was confirmed by a sensitive and rapid diagnostic polymerase chain reaction using the primers that amplify “A type inclusion” gene, and further, B5R gene of the virus was sequenced and compared with the corresponding sequences of other orthopoxviruses. The results showed high sequence homology of our isolates with other orthopoxviruses.
机译：本文描述了从sc疮病斑中分离出水牛痘病毒及其通过B5R基因测序的分子特征。在我们的研究中，牛和水牛的乳头和乳腺实质中发现了脓疱痘病，该疾病具有重要的人畜共患病重要性，因为与受感染动物密切接触的人的手，腿和面部也产生了类似的病斑。收集到的结ab材料通过绒膜绒毛膜途径和Vero细胞系在9-11日龄的鸡胚中进行病毒分离。通过使用扩增“ A型包涵体”基因的引物进行灵敏，快速的诊断性聚合酶链反应，确认了该病毒，然后对该病毒的B5R基因进行了测序，并与其他正痘病毒的相应序列进行了比较。结果表明我们的分离株与其他正痘病毒具有高度的序列同源性。

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