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Progesterone supplementation during multiple ovulation treatment in buffalo species (Bubalus bubalis)

机译:水牛种(Bubalus bubalis)在多次排卵治疗期间补充黄体酮

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The aim of this study was to evaluate the effect of exogenous progesterone supplementation on superovulatory response in buffaloes that has undergone a multiple ovulation program. Fourteen Mediterranean buffaloes were divided into two groups and received a 4-day decreasing dosage of an equal mixture of 500 IU of FSH and LH starting on day 8 of the cycle. In group A (n = 7) a progesterone-releasing intravaginal device was removed on day 8, whereas in group B (n = 7) it was left till day 10, when was administered. Eighty hours later, buffaloes were artificially inseminated and after 6 days they undergone uterine flushing. A higher (P < 0.05) number of corpora lutea (8.3 vs. 5.7) and embryo/flushing/buffalo (2.3 vs. 1.3) were recorded in group B vs. group A if responsive buffaloes are considered (n = 12) and the number of corpora lutea was highly correlated with the number of embryos (r = 0.65; P < 0.05). In conclusion, progesterone supplementation during the first 2 days of the superovulation treatment seems to enhance the recovery rate in buffalo species. A high ovulation rate, associated with a high number of corpora lutea, can represent a parameter for estimating embryo recovery.
机译:这项研究的目的是评估外源孕激素补充对经历多次排卵程序的水牛超排卵反应的影响。从周期的第8天开始,将14个地中海水牛分为两组,并接受4天的500 IU FSH和LH等量混合物的递减剂量。在A组(n = 7)中,在第8天摘除了孕激素释放型阴道内器械,而在B组(n = 7)中,将其给药至第10天。 80小时后,对牛进行人工授精,并在6天后对其进行子宫冲洗。如果考虑到反应性水牛(n = 12),则B组与A组相比,黄体(8.3 vs. 5.7)和胚胎/潮红/水牛(2.3 vs. 1.3)的数量更高(P <0.05)。黄体数目与胚胎数目高度相关(r = 0.65; P <0.05)。总之,在超排卵治疗的前两天补充黄体酮似乎可以提高水牛的恢复率。与大量黄体相关的高排卵率可以代表估计胚胎恢复的参数。

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