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The effect of estrus synchronization treatment on somatic cell count of transitional-anestrus local-Damascus cross breed goats’ milk

机译:发情同步化处理对过渡性发情本地大马士革杂交品种山羊乳体细胞计数的影响

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An experiment was conducted to examine the effect of estrus synchronization protocols and steroid hormones concentrations on somatic cell count (SCC) of transitional-anestrus local-Damascus cross goats’ milk. Fifty-six goats (2–4-year old) were randomly assigned to three groups: fluorogestone acetate (FGA, n = 19), FGA-Prostaglandin (FGA-PGF, n = 19) and control (n = 18) groups. Intravaginal sponge containing 40 mg FGA was inserted for 13 days and an injection of 600 IU equine chorionic gonadotropin (eCG) was administered for goats of FGA and FGA-PGF groups at the time of sponge removal (day 0). In addition, goats of FGA-PGF group were injected with 10 mg dinoprost tromethamine ( textPGF2a {text{PGF}}_{{2alpha }} ) on day 0. Five fertile local-Damascus cross bucks were turned-in with all goats on day 0. Blood and milk samples were collected from all goats on days -13 (beginning of experiment), -6, 0, 1, 2, 7, 13 and 20 (end of the experiment). Four-year old and second-parity goats had significantly higher (p < 0.05) SCC of both udder halves than 2- and 3-year old and first-parity goats, respectively. There was a significant effect (p < 0.05) for treatment and number of kids born in the last kidding season on SCC of both udder halves. Neither estradiol nor progesterone concentrations were correlated with SCC in goats in this experiment. The SCC of both udder halves and left udder halves in goats of the control and FGA groups, respectively, increased significantly (p < 0.05) after sponge removal and buck introduction when compared with day 0, with no differences in the FGA-PGF group. This increase in SCC of the control and FGA groups coincided with peak estrus behavior. However, SCC was far below the upper limit of the current standard for normal milk. In conclusion, induction of estrus with progestagen based programs and buck introduction may cause temporary significant increase in SCC. However, the SCC values during this period of temporary increase were still in the range of acceptable values for normal milk. With the current standards for SCC of 1,000,000/ml as legal limit for abnormal milk control programs in goats, estrus synchronization programs and the estrus status should not be considered when bulk-tank milk SCC is being investigated.
机译:进行了一项实验,研究了发情同步协议和类固醇激素浓度对过渡性发情本地大马士革杂交山羊奶体细胞计数(SCC)的影响。将五十六只山羊(2-4岁)随机分为三组:乙酸氟代石(FGA,n = 19),FGA-前列腺素(FGA-PGF,n = 19)和对照组(n = 18)。插入含40 mg FGA的阴道内海绵13天,并在去除海绵时(第0天)对FGA和FGA-PGF组的山羊注射600 IU马绒毛膜促性腺激素(eCG)。此外,在FGA-PGF组的山羊在第0天注射了10 mg地诺前列素三甲胺(textPGF 2a {text {PGF}} _ {{2alpha}})。五只肥沃的本地大马士革十字绣在第0天将所有山羊上交。在第-13天(实验开始),-6、0、1、2、7、13和20天(实验结束)从所有山羊采集血液和牛奶样本。 。四岁和第二胎山羊的乳房SCC均显着高于(p <0.05)两半山羊,分别高于2-3岁和第一胎山羊。在最后一个开玩笑的季节中,治疗和两个半数乳房的SCC都有显着影响(p <0.05)。在该实验中,雌二醇和孕酮的浓度均与山羊的SCC无关。对照组和FGA组山羊的半乳和左半乳的SCC与第0天相比分别在去除海绵和降压后显着增加(p <0.05),而FGA-PGF组没有差异。对照组和FGA组的SCC增加与发情高峰期相吻合。但是,SCC远远低于当前普通牛奶标准的上限。总之,以孕激素为基础的程序诱导发情和引入降压剂可能会导致SCC暂时性显着增加。但是,在此暂时增加期间的SCC值仍在正常牛奶可接受的范围内。由于目前的SCC标准为1,000,000 / ml,作为山羊异常乳汁控制程序的法定上限,因此在研究散装罐装牛奶SCC时,不应考虑发情同步程序和发情状态。

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