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Optimization of the procedure for counting the eggs of Fasciola gigantica in bovine faeces

机译：牛粪中费氏巨大孢子卵计数方法的优化

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This paper describes a method for counting eggs of F. gigantica in bovine faeces that optimizes the proportion of eggs recovered and the repeatability of estimates. The method uses 3 g of faeces suspended in 0.05% Tween 20. The suspension is passed through three 6 cm diameter sieves in tandem to remove fibrous debris, with respective apertures of 1 mm, 450 μm, and either 266 or 200 μm. The filtrate is allowed to sediment for 3 min in a conical flask; the sediment is recovered, then resuspended in 200 ml of 0.05% Tween 20 and allowed to sediment. After 3 min the sediment is washed in a sieve with an aperture of 53 μm, which retains the eggs. Eggs suspended in 15 ml of 1% methylene blue are counted using a dissecting microscope. Use of Tween 20 instead of water as the suspending agent for faeces gave a significant threefold increased the proportion of eggs recovered and reduced variability between repeated counts. This method is able to detect about one-third of the eggs present. It was concluded that the high proportion of F. gigantica eggs lost may be due to the presence of hydrophobic and covalent bonds on the eggs that bind them to debris, with which they are discarded.
机译：本文介绍了一种用于计算牛粪中巨巨耳卵数的方法，该方法可优化回收卵的比例和估计值的可重复性。该方法使用3 g的粪便悬浮在0.05％的Tween 20中。将悬浮液串联通过三个6 cm直径的筛网以除去纤维碎片，分别具有1 mm，450μm和266或200μm的孔径。将滤液在锥形烧瓶中沉淀3分钟；回收沉淀物，然后重悬于200 ml的0.05％Tween 20中，使其沉淀。 3分钟后，将沉淀物在孔径为53μm的筛子中洗涤，以保留卵。使用解剖显微镜对悬浮在15 ml 1％亚甲蓝中的卵进行计数。使用吐温20代替水作为粪便的悬浮剂，可显着提高三倍的卵回收率，并减少重复计数之间的变异性。这种方法能够检测出大约三分之一的卵。可以得出结论，丢失的巨无花果卵比例很高，可能是由于卵上存在疏水性和共价键，使它们与碎片结合在一起而被丢弃。

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