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首页> 外文期刊>Tropical Animal Health and Production >Evaluation of Diagnostic Tests for Trypanosoma evansi in Experimentally Infected Pigs and Subsequent Use in Field Surveys in North Vietnam and Thailand
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Evaluation of Diagnostic Tests for Trypanosoma evansi in Experimentally Infected Pigs and Subsequent Use in Field Surveys in North Vietnam and Thailand

机译：在越南北部和泰国的实验感染猪中的伊万氏锥虫诊断测试的评估及其随后的田间调查

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摘要

This study is concerned with the evaluation of established diagnostic tests for diagnosis of Trypanosoma evansi in pigs. The immune trypanolysis test (TL), card agglutination test (CATT), latex agglutination test (LATEX), enzyme-linked immunosorbent assay (ELISA), microhaematocrit centrifugation technique (MHCT) and mouse inoculation (MI) tests were initially evaluated in experimentally infected fattening pigs. All infected pigs were confirmed parasitologically positive with both MHCT and MI. Results of the serological assays indicated that the TL could be a reference test for the presence of RoTat 1.2 antibodies in pigs. The results of the CATT and LATEX were inconsistent with the TL while the ELISA results correlated with the TL results. The four serological assays were subsequently used in two field surveys in Vietnam and Thailand. Results of the two agglutination assays (CATT and LATEX) were not consistent and did not correlate with TL results. The ELISA at percentage positivity of 22 appeared to have good ability to discriminate between seropositive and seronegative animals. Of the 437 samples collected at smallholder pig premises in northern Vietnam, no positive pigs were detected with the TL test. In Thailand, 77 samples were collected from five farrowing farms with a history of surra. Two parasitologically positive sows were found and on each farm seropositive sows were detected.
机译：这项研究与已建立的诊断猪伊万氏锥虫病诊断方法的评估有关。最初在实验感染的动物中评估了免疫锥虫试验（TL），卡片凝集试验（CATT），乳胶凝集试验（LATEX），酶联免疫吸附测定（ELISA），微血球比重离心技术（MHCT）和小鼠接种（MI）试验肥猪。 MHCT和MI均确认所有感染的猪在寄生虫学上均为阳性。血清学检测结果表明，TL可以作为猪中RoTat 1.2抗体存在的参考测试。 CATT和LATEX的结果与TL不一致，而ELISA结果与TL的结果相关。随后在越南和泰国的两次现场调查中使用了这四种血清学检测方法。两种凝集试验（CATT和LATEX）的结果不一致，并且与TL结果不相关。阳性率为22的ELISA似乎具有很好的区分血清阳性和血清阴性动物的能力。在越南北部小农户猪场收集的437个样本中，TL测试未检测到阳性猪。在泰国，从五个有surra历史的分娩场中采集了77个样本。发现了两只寄生虫学阳性的母猪，并且在每个农场中都检测到血清阳性的母猪。

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  • 来源
    《Tropical Animal Health and Production》 |2005年第6期|457-467|共11页
  • 作者

    W. G. Holland; N. G. Thanh; T. T. Do; S. Sangmaneedet; B. Goddeeris; J. Vercruysse;

  • 作者单位

    Department of Virology Faculty of Veterinary Medicine Parasitology and Immunology Ghent UniversityNational Institute of Veterinary ResearchIntervet International;

    National Institute of Veterinary Research;

    National Institute of Veterinary Research;

    Department of Pathobiology Faculty of Veterinary Medicine Khon Kaen University;

    Department of Virology Faculty of Veterinary Medicine Parasitology and Immunology Ghent UniversityDepartment of Animal Science Faculty of Applied Bioscience and Engineering;

    Department of Virology Faculty of Veterinary Medicine Parasitology and Immunology Ghent University;

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  • 正文语种 eng
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  • 关键词

    diagnosis; Trypanosoma evansi; pigs; serology;

    机译：诊断;伊万氏锥虫;猪;血清学;

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