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Microbial contamination of contact lens storage cases and domestic tap water of contact lens wearers

机译:隐形眼镜存放盒的微生物污染和隐形眼镜佩戴者的生活自来水

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Contact lenses have been widely used as an alternative to spectacles both in developed and developing countries. However, under certain circumstances, adverse responses can occur during contact lens wear and several microorganisms—including bacteria, fungi, and free living amoebae—can cause several eye infections in wearers. Extended wear of contact lenses is the major risk factor of eye infections such as microbial keratitis, besides contaminated contact lens storage case, contaminated lens care solutions, and inaccurate contact lens handling. In this study, we collected contact lens storage case and domestic tap water samples from 50 asymptomatic contact lens wearers. We determined that total aerobic mesophilic bacteria were isolated in 45 (90 %), Gram negative rod bacteria were isolated in 20 (40 %), Pseudomonas spp. were isolated in 2 (4 %) and fungi were isolated in 18 (36 %) out of 50 contact lens storage cases. Free living amoebae were not detected in investigated contact lens storage cases. At the same time, out of 50, total aerobic mesophilic bacteria were isolated in 34 (68 %), fungi were isolated in 15 (30 %) and free living amoebae were isolated in 15 (30 %) domestic tap water samples. No Gram-negative rod bacteria and Pseudomonas spp. were detected in investigated water samples. Two contact lens case samples and two tap water samples were excluded from the analysis for Pseudomonas spp. for technical reasons. According to our findings, inadequate contact lens maintenance during lens wear may result in the contamination of contact lens storage cases. This situation can lead to severe eye infections in contact lens wearers over time.
机译:在发达国家和发展中国家,隐形眼镜已被广泛用作眼镜的替代品。但是,在某些情况下,配戴隐形眼镜期间可能会发生不良反应,几种微生物(包括细菌,真菌和自由活动的变形虫)会在佩戴者中引起几种眼部感染。隐形眼镜的长时间佩戴是造成眼部感染(如微生物角膜炎)的主要风险因素,此外还有污染的隐形眼镜存放盒,污染的隐形眼镜护理液以及不正确的隐形眼镜处理方法。在这项研究中,我们收集了50名无症状隐形眼镜佩戴者的隐形眼镜储存盒和生活用水样本。我们确定在45(90%)中分离出总有氧中温细菌,在20(40%)中分离出革兰阴性杆细菌(Pseudomonas spp)。在50个隐形眼镜存储盒中,有2个(4%)被分离出真菌,在18个(36%)中被分离了真菌。在调查过的隐形眼镜储存盒中未检出自由的活的变形虫。同时,从50份中分离出总有氧嗜温细菌(34%(68%)),从15份(30%)中分离出真菌,并从15份(30%)家庭自来水样品中分离出自由生活的变形虫。无革兰氏阴性杆状细菌和假单胞菌。在被调查的水样中被检测到。分析假单胞菌属的两个隐形眼镜盒样品和两个自来水样品。由于技术原因。根据我们的发现,隐形眼镜配戴期间隐形眼镜的维护不足会导致隐形眼镜存放盒的污染。随着时间的流逝,这种情况会导致隐形眼镜佩戴者严重的眼部感染。

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